Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort

Affiliations

¹ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: sonia.robinson@nih.gov.
² Glotech Inc., 1801 Research Blvd #605, Rockville, MD, 20850, United States. Electronic address: xuehuo.zeng@nih.gov.
³ Division of Biostatistics, School of Public Health, University of Minnesota, 420 Delaware St SE, Minneapolis, MN, 55455, United States. Electronic address: wguan@umn.edu.
⁴ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: sundaramr2@mail.nih.gov.
⁵ Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, 270 Farber Hall, Buffalo, NY, 14214, United States. Electronic address: pmendola@buffalo.edu.
⁶ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: putnickd@mail.nih.gov.
⁷ Departments of Pediatrics and Molecular & Human Genetics, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, 1100 Bates St., Ste. 5080, Houston, TX, 77030, United States. Electronic address: waterland@bcm.edu.
⁸ Departments of Pediatrics and Molecular & Human Genetics, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, 1100 Bates St., Ste. 5080, Houston, TX, 77030, United States. Electronic address: chathura.gunasekara@bcm.edu.
⁹ Department of Pediatrics and Department of Environmental Medicine, New York University School of Medicine, New York, NY, 10016, United States. Electronic address: kurunthachalam.kannan@nyulangone.org.
¹⁰ Department of Pediatrics and Department of Environmental Medicine, New York University School of Medicine, New York, NY, 10016, United States. Electronic address: gcj352535@163.com.
¹¹ Departments of Environmental Health Sciences, And Epidemiology and Biostatistics, University at Albany School of Public Health, 1 University Place, Rensselaer, NY, 12144, United States. Electronic address: ebell@albany.edu.
¹² Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: yeungedw@mail.nih.gov.

PMID: 33387539
PMCID: PMC7946760
DOI: 10.1016/j.envres.2020.110668

Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort

Sonia L Robinson et al. Environ Res. 2021 Mar.

. 2021 Mar:194:110668.

doi: 10.1016/j.envres.2020.110668. Epub 2020 Dec 30.

Authors

Affiliations

¹ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: sonia.robinson@nih.gov.
² Glotech Inc., 1801 Research Blvd #605, Rockville, MD, 20850, United States. Electronic address: xuehuo.zeng@nih.gov.
³ Division of Biostatistics, School of Public Health, University of Minnesota, 420 Delaware St SE, Minneapolis, MN, 55455, United States. Electronic address: wguan@umn.edu.
⁴ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: sundaramr2@mail.nih.gov.
⁵ Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, 270 Farber Hall, Buffalo, NY, 14214, United States. Electronic address: pmendola@buffalo.edu.
⁶ Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: putnickd@mail.nih.gov.
⁷ Departments of Pediatrics and Molecular & Human Genetics, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, 1100 Bates St., Ste. 5080, Houston, TX, 77030, United States. Electronic address: waterland@bcm.edu.
⁸ Departments of Pediatrics and Molecular & Human Genetics, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, 1100 Bates St., Ste. 5080, Houston, TX, 77030, United States. Electronic address: chathura.gunasekara@bcm.edu.
⁹ Department of Pediatrics and Department of Environmental Medicine, New York University School of Medicine, New York, NY, 10016, United States. Electronic address: kurunthachalam.kannan@nyulangone.org.
¹⁰ Department of Pediatrics and Department of Environmental Medicine, New York University School of Medicine, New York, NY, 10016, United States. Electronic address: gcj352535@163.com.
¹¹ Departments of Environmental Health Sciences, And Epidemiology and Biostatistics, University at Albany School of Public Health, 1 University Place, Rensselaer, NY, 12144, United States. Electronic address: ebell@albany.edu.
¹² Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 6710B Rockledge Drive, Bethesda, MD, 20892, United States. Electronic address: yeungedw@mail.nih.gov.

PMID: 33387539
PMCID: PMC7946760
DOI: 10.1016/j.envres.2020.110668

Abstract

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are persistent organic pollutants which may alter prenatal development, potentially through epigenetic modifications. Prior studies examining PFOS/PFOA and DNA methylation have relatively few subjects (n < 200) and inconsistent results. We examined relations of PFOA/PFOS with DNA methylation among 597 neonates in the Upstate KIDS cohort study. PFOA/PFOS were quantified in newborn dried blood spots (DBS) using high-performance liquid chromatography/tandem mass spectrometry. DNA methylation was measured using the Infinium MethylationEPIC BeadChip with DNA extracted from DBS. Robust linear regression was used to examine the associations of PFOA/PFOS with DNA methylation at individual CpG sites. Covariates included sample plate, estimated cell type, epigenetically derived ancestry, infant sex and plurality, indicators of maternal socioeconomic status, and prior pregnancy loss. In supplemental analysis, we restricted the analysis to 2242 CpG sites previously identified as Correlated Regions of Systemic Interindividual Variation (CoRSIVs) which include metastable epialleles. At FDR<0.05, PFOA concentration >90th percentile was related to DNA methylation at cg15557840, near SCRT2, SRXN1; PFOS>90th percentile was related to 2 CpG sites in a sex-specific manner (cg19039925 in GVIN1 in boys and cg05754408 in ZNF26 in girls). When analysis was restricted to CoRSIVs, log-scaled, continuous PFOS concentration was related to DNA methylation at cg03278866 within PTBP1. In conclusion, there was limited evidence of an association between high concentrations of PFOA/PFOS and DNA methylation in newborn DBS in the Upstate KIDS cohort. These findings merit replication in populations with a higher median concentration of PFOA/PFOS.

Keywords: Developmental origins of health and disease (DoHaD); Mother-child dyads; Newborn dried blood spots; Persistent organic pollutants.

Published by Elsevier Inc.

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Conflict of interest statement

Declaration of interests

☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

References

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Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort

Affiliations

Perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) and DNA methylation in newborn dried blood spots in the Upstate KIDS cohort

Authors

Affiliations

Abstract

Conflict of interest statement

References

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources