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. 2021 Jan;11(1):9-20.
doi: 10.21037/qims-20-517.

Repeatability of proton magnetic resonance spectroscopy of the brain at 7 T: effect of scan time on semi-localized by adiabatic selective refocusing and short-echo time stimulated echo acquisition mode scans and their comparison

Tomohisa Okada  1 , Hideto Kuribayashi  2 , Lana G Kaiser  3 , Yuta Urushibata  2 , Nouha Salibi  4 , Ravi Teja Seethamraju  4 , Sinyeob Ahn  4 , Dinh Ha Duy Thuy  1 , Koji Fujimoto  1 , Tadashi Isa  1
Affiliations

Repeatability of proton magnetic resonance spectroscopy of the brain at 7 T: effect of scan time on semi-localized by adiabatic selective refocusing and short-echo time stimulated echo acquisition mode scans and their comparison

Tomohisa Okada et al. Quant Imaging Med Surg. 2021 Jan.

Abstract

Background: Proton magnetic resonance spectroscopy (MRS) provides a unique opportunity for in vivo measurements of the brain's metabolic profile. Two methods of mainstream data acquisition are compared at 7 T, which provides certain advantages as well as challenges. The two representative methods have seldom been compared in terms of measured metabolite concentrations and different scan times. The current study investigated proton MRS of the posterior cingulate cortex using a semi-localized by adiabatic selective refocusing (sLASER) sequence and a short echo time (TE) stimulated echo acquisition mode (sSTEAM) sequence, and it compared their reliability and repeatability at 7 T using a 32-channel head coil.

Methods: Sixteen healthy subjects were prospectively enrolled and scanned twice with an off-bed interval between scans. The scan parameters for sLASER were a TR/TE of 6.5 s/32 ms and 32 and 48 averages (sLASER×32 and sLASER×48, respectively). The scan parameters for sSTEAM were a TR/TE of 4 s/5 ms and 32, 48, and 64 averages (sSTEAM4×32, sSTEAM4×48, and sSTEAM4×64, respectively) in addition to that with a TR/TE of 8 s/5 ms and 32 averages (sSTEAM8×32). Data were analyzed using LCModel. Metabolites quantified with Cramér-Rao lower bounds (CRLBs) >50% were classified as not detected, and metabolites quantified with mean or median CRLBs ≤20% were included for further analysis. The SNR, CRLBs, coefficient of variation (CV), and metabolite concentrations were statistically compared using the Shapiro-Wilk test, one-way ANOVA, or the Friedman test.

Results: The sLASER spectra for N-acetylaspartate + N-acetylaspartylglutamate (tNAA) and glutamate (Glu) had a comparable or higher SNR than sSTEAM spectra. Ten metabolites had lower CRLBs than prefixed thresholds: aspartate (Asp), γ-aminobutyric acid (GABA), glutamine (Gln), Glu, glutathione (GSH), myo-inositol (Ins), taurine (Tau), the total amount of phosphocholine + glycerophosphocholine (tCho), creatine + phosphocreatine (tCr), and tNAA. Performance of the two sequences was satisfactory except for GABA, for which sLASER yielded higher CRLBs (≥18%) than sSTEAM. Some significant differences in CRLBs were noted, but they were ≤2% except for GABA and Gln. Signal averaging significantly lowered CRLBs for some metabolites but only by a small amount. Measurement repeatability as indicated by median CVs was ≤10% for Gln, Glu, Ins, tCho, tCr, and tNAA in all scans, and that for Asp, GABA, GSH, and Tau was ≥10% under some scanning conditions. The CV for GABA according to sLASER was significantly higher than that according to sSTEAM, whereas the CV for Ins was higher according to sSTEAM. An increase in signal averaging contribute little to lower CVs except for Ins.

Conclusions: Both sequences quantified brain metabolites with a high degree of precision and repeatability. They are comparable except for GABA, for which sSTEAM would be a better choice.

Keywords: 7 Tesla (7 T); Cramér-Rao lower bound; Magnetic resonance spectroscopy (MRS); repeatability; semi-localized by adiabatic selective refocusing (sLASER); short echo time (TE) stimulated echo acquisition mode (sSTEAM).

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Conflict of interest statement

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure (available at http://dx.doi.org/10.21037/qims-20-517). TO serves as an unpaid editorial board member of Quantitative Imaging in Medicine and Surgery. TO reports grants from Siemens Healthcare K.K., during the conduct of the study; HK reports personal fees from Siemens Healthcare K.K. (Japan), during the conduct of the study; YU reports personal fees from Siemens Healthcare K.K. (Japan), during the conduct of the study; NS was a Siemens Research Collaboration manager in charge of MR Spectroscopy. NS retired in October 2017 and do not currently receive any salary from Siemens. RTS reports personal fees from Siemens Medical Solutions, USA Inc., during the conduct of the study; SA reports personal fees from Siemens Healthineers, during the conduct of the study. The other authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Voxel position is shown as a white box on the T1-weighted mid-sagittal image.
Figure 2
Figure 2
Representative MR spectra of (A) sLASER (TR/TE = 6,500/32 ms) and (B) sSTEAM (TR/TE = 4,000/5 ms) with 32 averages of the same subject. LCModel fits are drawn in red, and residuals are presented on the top of the spectra. sLASER, semi-localized by adiabatic selective refocusing; TR, time of repetition; TE, echo time.
Figure 3
Figure 3
SNR of (A) NAA (SNRNAA) and (B) glutamate (SNRGlu). Pulse sequences are sLASER with TR = 6.5 s (∆) and sSTEAM with TR = 4 s (○) and 8 s (●) with different numbers of averaging. SNR increased according to square root of scan time measured in seconds. In NAA, SNR was higher in sLASER than sSTEAM with TR = 4 s, but it was similar for Glu between the two sequences. The sSTEAM with TR = 8 s was lower in both metabolites. SNR, signal-to-noise ratio; NAA, N-acetylaspartate; sLASER, semi-localized by adiabatic selective refocusing; TR, time of repetition; STEAM, short echo time stimulated echo acquisition mode.
See this image and copyright information in PMC

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