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. 2020 Dec 18:7:544895.
doi: 10.3389/fmolb.2020.544895. eCollection 2020.

PCOLCE Is Potent Prognostic Biomarker and Associates With Immune Infiltration in Gastric Cancer

Aizhai Xiang  1   2 , Xia Lin  1   2 , Lvping Xu  1   2 , Honggang Chen  1   2 , Jufeng Guo  1   2 , Fang Zhou  1   2
Affiliations

PCOLCE Is Potent Prognostic Biomarker and Associates With Immune Infiltration in Gastric Cancer

Aizhai Xiang et al. Front Mol Biosci. .

Abstract

Background: The exact biological role of PCOLCE was not yet clear and there were few reports study the correlation of PCOLCE gene expression level with the occurrence and development of gastric cancer.

Methods: The expression of PCOLCE was analyzed by performing the Oncomine and Ualcan database. We evaluated the function of PCOLCE on clinical prognosis with the use of Kaplan-Meier plotter database. The relationship between PCOLCE and cancer immune in filtrates was researched by Tumor Immune Estimation Resource (TIMER) site database.

Results: PCOLCE significantly upregulated in gastric cancer patients compared to normal gastric samples. And the increased expression of PCOLCE mRNA was closely linked to shorter overall survival (OS), progress-free survival (PFS) in all gastric cancers. Besides, PCOLCE expression displayed a tight correlation with infiltrating levels of macrophages and dendritic cells (DCs) in gastric cancer. Moreover, PCOLCE expression was positively correlated with diverse immune marker sets in gastric cancer.

Conclusion: All the results above suggested that overexpression of PCOLCE indicated unfavorable prognosis in patients with gastric cancer. PCOLCE was correlated with immune infiltrating levels including those of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and DCs in gastric cancer patients. All the findings suggested that PCOLCE could be used as a prognostic biomarker for determining prognosis and immune infiltration in gastric cancer. Additionally, PCOLCE expression potentially contributed to the regulation of monocyte, M2 macrophage, Tfh, CD8 + T cell, TAM, Th1 cell Thus PCOLCE is a potential target for gastric cancer therapy and these preliminary findings require further study to determine whether PCOLCE-targeting reagents might be developed for clinical application in gastric cancer.

Keywords: PCOLCE; gastric cancer; immune infiltration; prognosis; prognostic biomarker.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
PCOLCE expression levels in different types of human cancers. (A) Increased or decreased PCOLCE in data sets of different cancers compared with normal tissues in the Oncomine database. (B) The expression of PCOLCE was higher in STAD cancer (stomach adenocarcinoma) compared to normal stomach adenocarcinoma tissues. Data derived from UALCAN database (***P < 0.001).
FIGURE 2
FIGURE 2
Kaplan–Meier survival curves comparing the high and low expression of PCOLCE in different types of cancer in the Kaplan–Meier plotter databases (A–H). (A–D) Survival curves of OS and PFS survival curves of gastric cancer and ovarian cancer. (E–H) Survival curves of OS and RFS survival curves of liver hepatocellular carcinoma and bladder cancer. OS, overall survival; DFS, progress-free survival; RFS, relapse-free survival.
FIGURE 3
FIGURE 3
Correlation of PCOLCE expression with immune infiltration level in STAD (stomach adenocarcinoma). PCOLCE expression is significant positive correlations with infiltrating levels of CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in STAD (stomach adenocarcinoma). While PCOLCE expression has no significant correlations with tumor purity.
See this image and copyright information in PMC

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