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. 2021 Jan 3;53(1):65.
doi: 10.1007/s11250-020-02491-4.

Effect of LPS and LTA stimulation on the expression of TLR-pathway genes in PBMCs of Akkaraman lambs in vivo

Esma Gamze Aksel  1 , Bilal Akyüz  2
Affiliations

Effect of LPS and LTA stimulation on the expression of TLR-pathway genes in PBMCs of Akkaraman lambs in vivo

Esma Gamze Aksel et al. Trop Anim Health Prod. .

Abstract

This is the first study investigating the changes in some gene expressions related to the TLR pathway in vivo in sheep. Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) molecules were administrated separately and in combination to the Akkaraman lambs via intranasal route. For this purpose, 28 lambs were distributed into four groups (LPS, LTA, LPS + LTA, and control, n = 7). Blood samples were collected to isolate the peripheral blood mononuclear cells (PBMCs) at 24 h and on day 7. Expression levels of TLR2, TLR4, MyD88, TRAF6, TNF-α, IL-1ß, IL-6, IL-10, NF-κß, and IFN-γ genes were determined by qRT-PCR. Increases were determined in the expression data of TLR2 [LPS (P < 0.05) and LTA + LPS (P < 0.01)], TLR4 [LTA + LPS (P < 0.05)], TNF-α, IL-10 [LTA + LPS (P < 0.05)], and IFN-γ genes in all groups in the mRNA expression analysis of PBMCs isolated at 24 h whereas decreases were determined in the expression levels of these genes on day 7. The combination of LPS + LTA stimulated lamb PBMCs more effectively than separate administration of LPS and LTA at 24 h. Therefore, this article may contribute to the understanding the host-pathogen interaction of respiratory-transmitted bacterial diseases concerning PBMCs at 24 h and on day 7. Also this study may contribute to the dose adjustment for bacterial vaccine studies in sheep. Experimental application doses will be helpful for in vivo and in vitro drug and vaccine development studies in the fields of pharmacology and microbiology.

Keywords: Gene expression; Lamb; Lipopolysaccharide; Lipoteichoic acid; Peripheral mononuclear blood cell.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Values of groups rectal temperature and blood pressure of 24 h and day 7. *P < 0.05; **P < 0.01; ***P < 0.001
Fig. 2
Fig. 2
Expression levels (mRNA expression level) of 24 h and day 7 of TLR2 and TLR4 genes examined in all groups. *P < 0.05; **P < 0.01
Fig. 3
Fig. 3
Expression levels (mRNA expression level) of the 24 h and day 7 signaling MyD88 and TRAF6 genes examined in all groups. *P < 0.05; **P < 0.01
Fig. 4
Fig. 4
24-h expression levels (mRNA expression level) of NF-κβ, TNFα, IL-1β, IL-10, IFN-γ genes examined in all groups. *P < 0.05; **P < 0.01
Fig. 5
Fig. 5
Day 7 expression levels (mRNA expression level) of NF-κβ, TNFα, IL-1β, IL-10, IFN-γ genes examined in all groups. *P < 0.05; **P < 0.01
See this image and copyright information in PMC

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