Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan;48(1):657-664.
doi: 10.1007/s11033-020-06113-y. Epub 2021 Jan 3.

Identification and response of cytochrome P450 genes in the brackish water flea Diaphanosoma celebensis after exposure to benzo[α]pyrene and heavy metals

Jeonghoon Han  1 , Kyun-Woo Lee  2
Affiliations

Identification and response of cytochrome P450 genes in the brackish water flea Diaphanosoma celebensis after exposure to benzo[α]pyrene and heavy metals

Jeonghoon Han et al. Mol Biol Rep. 2021 Jan.

Abstract

The cytochrome P450 (CYP) enzyme family is extensive; these enzymes participate in phase I enzyme metabolism and are involved in xenobiotic detoxification in all living organisms. Despite their significance in xenobiotic detoxification, little is known about the species-specific comparison of CYPs and their molecular responses in aquatic invertebrates. We identified 31 CYPs in the brackish water flea Diaphanosoma celebensis via thorough exploration of transcriptomic databases and measured the transcript profiles of 9 CYPs (within full sequences) in response to benzo[α]pyrene (B[α]P) and two heavy metals (cadmium [Cd] and copper [Cu]). Through phylogenetic analysis, the CYPs were separated and clustered into four clans: mitochondrial, CYP2, CYP3, and CYP4. The expression of 9 CYPs were differentially modulated (up- and/or downregulated) in response to B[α]P, Cd, and Cu. In particular, CYP370A15 was significantly upregulated in response to B[α]P, Cd, and Cu, suggesting that the identified CYPs are involved in xenobiotic detoxification and are useful as biomarkers in response to B[α]P, Cd, and Cu. This study aimed to comprehensively annotate cladoceran CYPs; our results will add to the existing knowledge on the potential roles of CYPs in xenobiotic detoxification in cladocerans.

Keywords: Cytochrome P450s; Detoxification; Diaphanosoma celebensis; Heavy metals; Polycyclic aromatic hydrocarbon; Transcriptional modulation.

PubMed Disclaimer

References

    1. Degtyarenko KN, Archakov AI (1993) Molecular evolution of P450 super-family and P450-containing monooxygenase systems. FEBS Lett 332:1–8 - PubMed
    1. Nelson DR, Koymans L, Kamataki T, Stegeman JJ, Feyereisen R, Waxman DJ, Waterman MR, Gotoh O, Coon MJ, Estabrook RW, Gunsalus IC, Nebert DW (1996) The P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6:1–42 - PubMed
    1. Chaty S, Rodius F, Vasseur P (2004) A comparative study of the expression of CYP1A and CYP4 genes in aquatic invertebrates (freshwater mussel, Unio tumidus) and vertebrates (rainbow trout, Oncorhynchus mykiss). Aquat Toxicol 69:81–93 - PubMed
    1. Feyereisen R (2006) Evolution of insect P450. Biochem. Soc Trans 34:1252–1255
    1. Snyder MJ (2000) Cytochrome P450 enzymes in aquatic invertebrates: recent advances and future directions. Aquat Toxicol 48:529–547 - PubMed

MeSH terms

LinkOut - more resources