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. 2021 Aug;236(8):5742-5756.
doi: 10.1002/jcp.30258. Epub 2021 Jan 3.

Effects and mechanism of lncRNA-27785.1 that regulates TGF-β1 of Sika deer on antler cell proliferation

Dan-Yang Chen  1 , Yan-Jun Li  1 , Ren-Feng Jiang  1 , Yu-Ting Li  1 , Jiang Feng  1 , Wei Hu  1
Affiliations

Effects and mechanism of lncRNA-27785.1 that regulates TGF-β1 of Sika deer on antler cell proliferation

Dan-Yang Chen et al. J Cell Physiol. 2021 Aug.

Abstract

Transforming growth factor (TGF-β) plays an important role in the development of deer antlers. The purpose of this study was to investigate the role of long noncoding RNA in the transcriptional regulation of TGF-β1 and its relationship with the proliferation and differentiation of antler chondrocytes. High-throughput sequencing was used to screen lncRNAs related to TGF-β1. Next, the overexpression plasmid and interference sequence of target lncRNA27785.1 were constructed and transfected into chondrocytes. We found that lncRNA27785.1 inhibited the proliferation and migration of chondrocytes and delayed the transition of cells from G1 to S phase. qRT-PCR and Western blot analysis indicated that the overexpression of lncRNA27785.1 may downregulate mRNA and protein expression of TGF-BR2, Smad3, pSmad3, and Smad4. Our findings highlight lncRNA27785.1 as an inhibitor of chondrocytes proliferation and differentiation by negatively regulating the TGF-β/Smad signaling pathway; this implicates an important regulatory role for long noncoding RNA in the regeneration of antler.

Keywords: Sika; TGF-β1; antler; cell proliferation; long noncoding RNA.

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