Microbial characteristics of nitrifiers, denitrifiers and anammox bacteria on different support media to treat space mission wastewater

Abstract

Biomass attachment and growth are important factors for the startup and stability of fixed-film biological reactors being proposed to recycle wastewater for potable water use in manned space activity. Eight different biofilm support media commonly used in wastewater treatment plants, aquaculture, and aquariums were compared for their relative ability to support attachment and growth of nitrifiers, denitrifiers, and anaerobic ammonia oxidizing (anammox) bacteria biomass. Accumulated total biomass was determined by comparing dry weight of each media before and after culturing of biomass. Fluorescence In-Situ Hybridization (FISH) analysis was used to quantify the proportion and relative activity of each organism group on each media. Measurements of dry biomass normalized to several media properties showed polyether polyurethane foam to have the highest extent of specific biomass attachment and colonization. Six of the eight media were able to sustain a population of anammox bacteria that was more abundant than the other cohorts.

Keywords: Biofilm support media; Biomass growth; Early planetary base wastewater; Fluorescence in-situ hybridization.