Best practices in plant cytometry

David Galbraith^{1

2}, João Loureiro³, Ioanna Antoniadi⁴, Jillian Bainard⁵, Petr Bureš⁶, Petr Cápal⁷, Mariana Castro³, Sílvia Castro³, Martin Čertner^{8

9}, Dora Čertnerová⁸, Zuzana Chumová^{8

9}, Jaroslav Doležel⁷, Debora Giorgi¹⁰, Brian C Husband¹¹, Filip Kolář^{8

9}, Petr Koutecký¹², Paul Kron¹¹, Ilia J Leitch¹³, Karin Ljung⁴, Sara Lopes³, Magdalena Lučanová^{9

12}, Sergio Lucretti¹⁰, Wen Ma^{1

2}, Susanne Melzer^{14

15}, István Molnár⁷, Ondřej Novák^{4

16}, Nicole Poulton¹⁷, Vladimír Skalický¹⁶, Elwira Sliwinska¹⁸, Petr Šmarda⁶, Tyler W Smith¹⁹, Guiling Sun^{1

2}, Pedro Talhinhas²⁰, Attila Tárnok^{15

21

22}, Eva M Temsch²³, Pavel Trávníček⁹, Tomáš Urfus⁸

Affiliations

¹ School of Plant Sciences, BIO5 Institute, Arizona Cancer Center, Department of Biomedical Engineering, University of Arizona, Tucson, Arizona, USA.
² State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, Henan University, School of Life Sciences, State Key Laboratory of Crop Stress Adaptation and Improvement, Kaifeng, China.
³ Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
⁴ Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
⁵ Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, Saskatchewan, Canada.
⁶ Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, CZ, Czech Republic.
⁷ Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic.
⁸ Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.
⁹ Czech Academy of Sciences, Institute of Botany, Průhonice, Czech Republic.
¹⁰ Green Biotechnology Laboratory, Biotechnology and Agroindustry Division, Casaccia Research Center, ENEA - Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, Italy.
¹¹ Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada.
¹² Department of Botany, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
¹³ Department of Comparative Plant and Fungal Biology, Royal Botanic Gardens, Richmond, UK.
¹⁴ Clinical Trial Centre Leipzig, University Leipzig, Leipzig, Germany.
¹⁵ LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, Germany.
¹⁶ Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University, Olomouc, Czech Republic.
¹⁷ Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA.
¹⁸ Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.
¹⁹ Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
²⁰ LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
²¹ Department of Precision Instruments, Tsinghua University, Beijing, China.
²² Department for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
²³ Department of Botany and Biodiversity Research, University of Vienna, Vienna, Austria.

PMID: 33398930
DOI: 10.1002/cyto.a.24295

Free article

Editorial

Best practices in plant cytometry

David Galbraith et al. Cytometry A. 2021 Apr.

Free article

. 2021 Apr;99(4):311-317.

doi: 10.1002/cyto.a.24295. Epub 2021 Jan 4.

Authors

Affiliations

¹ School of Plant Sciences, BIO5 Institute, Arizona Cancer Center, Department of Biomedical Engineering, University of Arizona, Tucson, Arizona, USA.
² State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, Henan University, School of Life Sciences, State Key Laboratory of Crop Stress Adaptation and Improvement, Kaifeng, China.
³ Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
⁴ Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
⁵ Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, Saskatchewan, Canada.
⁶ Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, CZ, Czech Republic.
⁷ Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic.
⁸ Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.
⁹ Czech Academy of Sciences, Institute of Botany, Průhonice, Czech Republic.
¹⁰ Green Biotechnology Laboratory, Biotechnology and Agroindustry Division, Casaccia Research Center, ENEA - Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, Italy.
¹¹ Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada.
¹² Department of Botany, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
¹³ Department of Comparative Plant and Fungal Biology, Royal Botanic Gardens, Richmond, UK.
¹⁴ Clinical Trial Centre Leipzig, University Leipzig, Leipzig, Germany.
¹⁵ LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, Germany.
¹⁶ Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University, Olomouc, Czech Republic.
¹⁷ Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA.
¹⁸ Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.
¹⁹ Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
²⁰ LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
²¹ Department of Precision Instruments, Tsinghua University, Beijing, China.
²² Department for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
²³ Department of Botany and Biodiversity Research, University of Vienna, Vienna, Austria.

PMID: 33398930
DOI: 10.1002/cyto.a.24295

No abstract available

Keywords: best practices; chromosome analysis; cytometry; nuclear suspensions; plant sciences; protoplasts and organelle analysis; single cell suspensions.

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References

REFERENCES

1. Shapiro HM. Practical flow cytometry. 4th ed. New York: John Wiley & Sons Inc.; 2003 736 p.
1. Touchell DH, Palmer IE, Ranney TG. In vitro ploidy manipulation for crop improvement. Front Plant Sci. 2020;11:722.
1. Leitch I, Johnston E, Pellicer J, Hidalgo O, Bennett M. Plant DNA C-values Database (Release 7.1). 2020. https://cvalues.science.kew.org/.
1. Pellicer J, Leitch IJ. The plant DNA C-values database (release 7.1): an updated online repository of plant genome size data for comparative studies. New Phytol. 2020;226:301-5.
1. Appels R, Eversole K, Feuillet C, Keller B, Rogers J, Stein N, et al. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science. 2018;36(1):eaar7191.

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Best practices in plant cytometry

Affiliations

Best practices in plant cytometry

Authors

Affiliations

References

REFERENCES

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MeSH terms

LinkOut - more resources

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