Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 3;77(3):e29-e31.
doi: 10.1161/HYPERTENSIONAHA.120.16724. Epub 2021 Jan 5.

Concerns on the Specificity of Commercial ELISAs for the Measurement of Angiotensin (1-7) and Angiotensin II in Human Plasma

Mark C Chappell  1 , Nancy T Pirro  1 , Andrew M South  1   2 , TanYa M Gwathmey  1
Affiliations

Concerns on the Specificity of Commercial ELISAs for the Measurement of Angiotensin (1-7) and Angiotensin II in Human Plasma

Mark C Chappell et al. Hypertension. .
No abstract available

Keywords: coronavirus; heart failure; kidney diseases; pandemics; renin-angiotensin system.

PubMed Disclaimer

Conflict of interest statement

None.

Figures

Figure.
Figure.
Comparison of Ang (1–7) (angiotensin (1–7)) and Ang II (angiotensin II) peptide levels in pooled human plasma by ELISA and radioimmunoassay (RIA) methods. A, Ang (1–7) content in plasma (direct) or extracted plasma (extracted) by ELISA or in 1 mL extracted plasma by RIA. Direct plasma measurement with 50 pg/mL of ELISA Ang (1–7) added (direct + A7) or extracted plasma with 50 pg/mL of ELISA Ang (1–7) added (extracted + A7) by ELISA. B, Ang II content in plasma (direct) and extracted plasma (extracted) by ELISA or extracted plasma by RIA. Direct plasma with 50 pg/mL of ELISA Ang II added (direct + AII) or extracted plasma with 500 pg/mL of ELISA Ang II added (extracted + AII) by ELISA. Data are means±SEM, N=3; α P<0.05 vs direct or RIA, β P<0.05 vs direct.
See this image and copyright information in PMC

References

    1. South AM, Diz DI, Chappell MC. COVID-19, ACE2, and the cardiovascular consequences. Am J Physiol Heart Circ Physiol. 2020;318:H1084–H1090. doi: 10.1152/ajpheart.00217.2020. - PMC - PubMed
    1. Sparks MA, South AM, Badley AD, Baker-Smith CM, Batlle D, Bozkurt B, Cattaneo R, Crowley SD, Dell’Italia LJ, Ford AL, et al. Severe acute respiratory syndrome coronavirus 2, COVID-19, and the renin-angiotensin system: pressing needs and best research practices. Hypertension. 2020;76:1350–1367. doi: 10.1161/HYPERTENSIONAHA.120.15948 - PMC - PubMed
    1. Liu Y, Yang Y, Zhang C, Huang F, Wang F, Yuan J, Wang Z, Li J, Li J, Feng C, et al. Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury. Sci China Life Sci. 2020;63:364–374. doi: 10.1007/s11427-020-1643-8 - PMC - PubMed
    1. PubMed numbers of reports using Ang-(1-7) or Ang II Cloud Clone ELISAs: 25804096;26740254;26867655;26963622;27391973;28187460;28367863;28433566;28656296;28895159;28919108;29240788;29590257;29752040;29684507;29846109;29977313;30289294;31586210;32756181.
    1. Brosnihan KB, Chappell MC. Measurement of angiotensin peptides: HPLC-RIA. Methods Mol Biol. 2017;1527:81–99. doi: 10.1007/978-1-4939-6625-7_7 - PMC - PubMed

Publication types