Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec;14(9):803-808.
doi: 10.1049/iet-nbt.2020.0085.

Nano-encapsulated Tinospora cordifolia (Willd.) using poly (D, L-lactide) nanoparticles educe effective control in streptozotocin-induced type 2 diabetic rats

Ragavee Ambalavanan  1 , Arul Daniel John  1 , Asha Devi Selvaraj  2
Affiliations

Nano-encapsulated Tinospora cordifolia (Willd.) using poly (D, L-lactide) nanoparticles educe effective control in streptozotocin-induced type 2 diabetic rats

Ragavee Ambalavanan et al. IET Nanobiotechnol. 2020 Dec.

Abstract

The therapeutics for type 2 diabetes mellitus has emerged in the current century towards nanomedicine incorporated with plant active compounds. In this study, Tinospora cordifolia loaded poly (D, L-lactide) (PLA) nanoparticles (NPs) were evaluated in vivo for their anti-hyperglycemic potency towards streptozotocin-induced type 2 diabetic rats. T. cordifolia loaded PLA NPs were synthesised by the double solvent evaporation method using PLA polymer. The NPs were then characterised and administrated orally for 28 successive days to streptozotocin-induced diabetic rats. The PLA NPs had significant anti-diabetic effects which were equal to the existing anti-diabetic drug glibenclamide. The antidiabetic activity is due to the synergism of compounds present in stem extract of the plant which reduced the side effects and anti-diabetic.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
In vitro analysis of TC loaded PLA NPs (a) Percentage haemolysis of TC‐loaded PLA NPs, (b) Cell viability of TC‐loaded PLA NPs
Fig. 2
Fig. 2
Acute toxicity study (a) Effects of body weight in rats, (b) Histological examination of rat liver. Tissues were stained with haematoxylin and eosin (100×)
Fig. 3
Fig. 3
In vivo antidiabetic study (a) Effects of body weight in rats, (b) Effects of fasting blood glucose level
Fig. 4
Fig. 4
Quantitative determination of rat insulin concentration by ELISA
Fig. 5
Fig. 5
Histopathological examinations (a) Histological examination of rat liver. Tissues were stained with haematoxylin and eosin (100×), (b) Histological examination of rat pancreas. Tissues were stained with haematoxylin and eosin (100×)
See this image and copyright information in PMC

References

    1. Owens D.R., Zinman B., Bolli G.B.: ‘Insulins today and beyond’, Lancet, 2001, 358, (9283), pp. 739–746 - PubMed
    1. Bener A., Saleh N.M., Al‐Hamaq A.: ‘Prevalence of gestational diabetes and associated maternal and neonatal complications in a fast‐developing community: global comparisons’, Int. J. Womens Health, 2011, 3, pp. 367–373 - PMC - PubMed
    1. Diabetes Care : ‘Diagnosis and classification of diabetes mellitus’ (American Diabetes Association, USA, 2004), pp. s5–s10 - PubMed
    1. Oloyede H.O.B., Bello T.O., Ajiboye T.O. et al.: ‘Antidiabetic and antidyslipidemic activities of aqueous leaf extract of dioscoreophyllum cumminsii (Stapf) Diels in alloxan‐induced diabetic rats’, J. Ethnopharmacol., 2015, 166, pp. 313–322 - PubMed
    1. Atal C.K., Sharma M.L., Kaul A. et al.: ‘Immunomodulating agents of plant origin. I: preliminary screening’, J. Ethnopharmacol., 1986, 18, pp. 133–141 - PubMed