Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar;56(3):498-510.
doi: 10.1111/rda.13888. Epub 2021 Jan 18.

Melatonin and CIDR improved the follicular and luteal haemodynamics, uterine and ovarian arteries vascular perfusion, ovarian hormones and nitric oxide in cyclic cows

Elshymaa A Abdelnaby  1 , Amal M Abo El-Maaty  2
Affiliations

Melatonin and CIDR improved the follicular and luteal haemodynamics, uterine and ovarian arteries vascular perfusion, ovarian hormones and nitric oxide in cyclic cows

Elshymaa A Abdelnaby et al. Reprod Domest Anim. 2021 Mar.

Abstract

This study hypothesizes that melatonin with exogenous progesterone (CIDR) can improve follicular, luteal, ovarian and uterine haemodynamic of heat-stressed cows. Holstein cows (N = 12) studied for two spontaneous oestrous cycles during winter then divided equally during summer into the CIDR group received CIDR for 7 days and the melatonin group (Mel) received three injections of melatonin (75 mg/head) at the CIDR insertion, removal and ovulation days. Blood samples were collected to assay oestradiol (E2), progesterone (P4) and nitric oxide (NO). On day 0 (Ovulation), Mel had more small follicles (p < .05), higher ipsilateral and contralateral ovarian arteries (Ov.A.) peak systolic velocity (PSV), higher ipsilateral uterine artery (Ut.A.) PSV (p = .031) and blood flow volume (BFV), also Mel elevated contralateral Ut.A. PSV and BFV (p < .0001) but lowered contra Ut.A. pulsatility index (PI, p < .0001), E2 (p < .01) and NO (p < .0001). Mel increased the corpus luteum diameter (CL, p < .001), coloured area (p < .007) and P4 (p < .0001) on day 5 and reduced them (p < .05; p < .01) on Day 14. On day 10, Mel obtained CL diameter (p < .03) and coloured area (p < .002) of spontaneous that was higher than CIDR and decreased P4 (p < .003). Mel increased CL diameter, area and coloured area and decreased them thereafter. Mel increased the ipsilateral ovarian and uterine arteries PSV and BFV before ovulation and until day 8. Mel increased P4 and decreased NO until days 6 and 14. In conclusion, the improvement in follicular, luteal, ovarian and uterine haemodynamic and the decrease of NO production proved our hypothesis Melatonin doses higher than 75 mg/head is recommended to improve the heat-stressed cow's fertility.

Keywords: blood flow pattern; cows; follicular-luteal haemodynamic; melatonin.

PubMed Disclaimer

References

REFERENCES

    1. Abdelnaby, E. A. (2020a). Higher doses of Melatonin affect ovarian and middle uterine arteries vascular blood flow and induce oestrus earlier in acyclic ewes. Reproduction in Domestic Animals, 55(7), 763-769. https://doi.org/10.1111/rda.13678
    1. Abdelnaby, E. A. (2020b). Hemodynamic changes evaluated by Doppler ultrasonographic technology in the ovaries and uterus of dairy cattle after the puerperium. Reproductive Biology, 20, 202-209. https://doi.org/10.1016/j.repbio.2020.03.001
    1. Abdelnaby, E. A., & Abo El-Maaty, A. M. (2017a). Dynamics of follicular blood flow, antrum growth, and angiogenic mediators in mares from deviation to ovulation. Journal of Equine Veterinary Science, 55, 51-59. https://doi.org/10.1016/j.jevs.2017.04.003
    1. Abdelnaby, E. A., & Abo El-Maaty, A. M. (2017b). Luteal blood flow and growth in correlation to circulating angiogenic hormones after spontaneous ovulation in mares. Bulgarian Journal of Veterinary Medicine, 20, 97-109. https://doi.org/10.15547/bjvm.998
    1. Abdelnaby, E. A., & Abo El-Maaty, A. M. (2020). Effect of the side of ovulation on the uterine morphometry, blood flow, progesterone, oestradiol and nitric oxide during spontaneous and induced oestrus in lactating dairy cows. Reproduction in Domestic Animals, 55(7), 851-860. https://doi.org/10.1111/rda.13693

LinkOut - more resources