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. 2021 Mar;16(1):4-6.
doi: 10.1007/s11481-020-09975-y. Epub 2021 Jan 6.

SARS-COV2 Alters Blood Brain Barrier Integrity Contributing to Neuro-Inflammation

Jessica L Reynolds  1 , Supriya D Mahajan  2
Affiliations

SARS-COV2 Alters Blood Brain Barrier Integrity Contributing to Neuro-Inflammation

Jessica L Reynolds et al. J Neuroimmune Pharmacol. 2021 Mar.
No abstract available

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Figures

Fig. 1
Fig. 1
Our experimental paradigm included treating primary human BMVEC (Cat# ACBRI-376) with 5 μl (0.5 μg/ml) media (0.1 μg/μl stock) recombinant Spike protein from SARS-Related Coronavirus 2, Wuhan-Hu-1 (Cat # NR-52308; Lot: 70034410 BEI resources Inc) or 5 μl of Heat Inactivated SARS-Coronavirus 2, Isolate USA-WA1/2020, (Cat # NR-52286, Lot: 70033548; Pre-Inactivation Titer by TCID50 Assay in Vero E6 Cells = 1.6 × 105 TCID50 per mL, BEI resources Inc) for 24–48 h, followed by examining ACE2 receptor expression, BBB integrity, TJ protein gene expression levels and levels of pro-inflammatory cytokines. a Immunofluorescent staining of ACE-2 receptor on BMVEC; b Measurement of TEER; c-f ZO-1, ZO-2, Claudin-5 and JAM-2 gene expression data as analyzed by QPCR
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