Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Weijian Zong^#^{1

2

3}, Runlong Wu^#⁴, Shiyuan Chen¹, Junjie Wu⁵, Hanbin Wang⁶, Zhe Zhao⁷, Guoqing Chen¹, Rui Tu¹, Danlei Wu⁸, Yanhui Hu⁸, Yangyang Xu⁸, Yao Wang⁶, Zhuoli Duan⁹, Haitao Wu⁷, Yunfeng Zhang¹⁰, Jue Zhang^{5

6}, Aimin Wang^{11

12}, Liangyi Chen^{13

14

15}, Heping Cheng^{1

16}

Affiliations

¹ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
² Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing, China.
³ Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway.
⁴ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. rlwu@pku.edu.cn.
⁵ College of Engineering, Peking University, Beijing, China.
⁶ Academy of Advanced Interdisciplinary Study, Peking University, Beijing, China.
⁷ Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, China.
⁸ Beijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
⁹ Department of Neurology, Southern Medical University, Guangzhou, China.
¹⁰ Department of Electronics, Peking University, Beijing, China.
¹¹ Department of Electronics, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹² State Key Laboratory of Advanced Optical Communication System and Networks, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹³ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. lychen@pku.edu.cn.
¹⁴ PKU-IDG/McGovern Institute for Brain Research, Beijing, China. lychen@pku.edu.cn.
¹⁵ Beijing Academy of Artificial Intelligence, Beijing, China. lychen@pku.edu.cn.
¹⁶ Research Unit of Mitochondria in Brain Diseases, Chinese Academy of Medical Sciences, PKU-Nanjing Institute of Translational Medicine, Nanjing, China.

^# Contributed equally.

PMID: 33408404
DOI: 10.1038/s41592-020-01024-z

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Weijian Zong et al. Nat Methods. 2021 Jan.

. 2021 Jan;18(1):46-49.

doi: 10.1038/s41592-020-01024-z. Epub 2021 Jan 6.

Authors

Affiliations

¹ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
² Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing, China.
³ Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway.
⁴ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. rlwu@pku.edu.cn.
⁵ College of Engineering, Peking University, Beijing, China.
⁶ Academy of Advanced Interdisciplinary Study, Peking University, Beijing, China.
⁷ Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, China.
⁸ Beijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
⁹ Department of Neurology, Southern Medical University, Guangzhou, China.
¹⁰ Department of Electronics, Peking University, Beijing, China.
¹¹ Department of Electronics, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹² State Key Laboratory of Advanced Optical Communication System and Networks, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹³ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. lychen@pku.edu.cn.
¹⁴ PKU-IDG/McGovern Institute for Brain Research, Beijing, China. lychen@pku.edu.cn.
¹⁵ Beijing Academy of Artificial Intelligence, Beijing, China. lychen@pku.edu.cn.
¹⁶ Research Unit of Mitochondria in Brain Diseases, Chinese Academy of Medical Sciences, PKU-Nanjing Institute of Translational Medicine, Nanjing, China.

^# Contributed equally.

PMID: 33408404
DOI: 10.1038/s41592-020-01024-z

Erratum in

Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging.
Zong W, Wu R, Chen S, Wu J, Wang H, Zhao Z, Chen G, Tu R, Wu D, Hu Y, Xu Y, Wang Y, Duan Z, Wu H, Zhang Y, Zhang J, Wang A, Chen L, Cheng H. Zong W, et al. Nat Methods. 2021 Feb;18(2):220. doi: 10.1038/s41592-021-01066-x. Nat Methods. 2021. PMID: 33479525 No abstract available.

Abstract

We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm³ at a lateral resolution of ~1 μm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.

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References

1. Ghosh, K. K. et al. Miniaturized integration of a fluorescence microscope. Nat. Methods 8, 871–878 (2011). - DOI
1. Liberti, W. A., Perkins, L. N., Leman, D. P. & Gardner, T. J. An open source, wireless capable miniature microscope system. J. Neural Eng. 14, 045001 (2017). - DOI
1. Zhang, L. et al. Miniscope GRIN lens system for calcium imaging of neuronal activity from deep brain structures in behaving animals. Curr. Protoc. Neurosci. 86, e56 (2019). - DOI
1. Chen, S. et al. Miniature fluorescence microscopy for imaging brain activity in freely-behaving animals. Neurosci. Bull. https://doi.org/10.1007/s12264-020-00561-z (2020).
1. Wallace, D. J. & Kerr, J. N. D. Circuit interrogation in freely moving animals. Nat. Methods 16, 9–11 (2019). - DOI

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Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Affiliations

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Authors

Affiliations

Erratum in

Abstract

References

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LinkOut - more resources

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