. 2021 Jan 7;22(1):34.

doi: 10.1186/s12864-020-07331-1.

Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

Yuqi Wang^{1

2}, Can Yu^{1

2}, Yukun Cheng^{1

2}, Fangjie Yao^{1

2}, Li Long^{1

2}, Yu Wu^{1

2}, Jing Li^{1

2}, Hao Li¹, Jirui Wang^{1

2}, Qiantao Jiang^{1

2}, Wei Li³, Zhien Pu³, Pengfei Qi¹, Jian Ma¹, Mei Deng¹, Yuming Wei^{1

2}, Xianming Chen⁴, Guoyue Chen^{1

2}, Houyang Kang^{5

6}, Yunfeng Jiang⁷, Youliang Zheng^{8

9}

Affiliations

¹ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
² State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
³ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
⁴ US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit; and Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA.
⁵ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn.
⁶ State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn.
⁷ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. jiangyunfeng@sicau.edu.cn.
⁸ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn.
⁹ State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn.

PMID: 33413106
PMCID: PMC7791647
DOI: 10.1186/s12864-020-07331-1

Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

Yuqi Wang et al. BMC Genomics. 2021.

. 2021 Jan 7;22(1):34.

doi: 10.1186/s12864-020-07331-1.

Authors

Affiliations

¹ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
² State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
³ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
⁴ US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit; and Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA.
⁵ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn.
⁶ State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn.
⁷ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. jiangyunfeng@sicau.edu.cn.
⁸ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn.
⁹ State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn.

PMID: 33413106
PMCID: PMC7791647
DOI: 10.1186/s12864-020-07331-1

Abstract

Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment.

Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99-23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci.

Conclusions: Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

Keywords: Chinese wheat landrace; GWAS; Southern China; Stripe rust resistance.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

**Fig. 1**
Box plot, violin plot and raw data points distributions of IT (a) evaluated in the seedling stage for CYR32 and CYR34; At the adult plant stage, IT (b), FDS (c) and the AUDPC (d) evaluated against *Pst* of mixed races in five environments. Tests at Chongzhou from the year 2016 to 2018 was referred to as CZ16, CZ17 and CZ18; at Mianyang from the year 2016 to 2017 referred to as MY16 and MY17, respectively

**Fig. 2**
The MLM Manhattan plot of stripe rust resistance significantly associated markers. The horizontal line shows the genome-wide significant threshold –log10(P) value of 3.0. The associated MTAs for IT of CYR32, CYR34 with seedling resistance, IT, FDS and AUDPC based on the BLUP from the inner circle to the outer circle

**Fig. 3**
The position of the potentially novel QTL on chromosomes 1B, 2B, 3B and 6A in this study. QTL marked as red color on the left side of chromosomes were the potentially new QTL in this study. The reported genes and QTL were marked as black color and mapped on the left and right side of the chromosomes separately

**Fig. 4**
Regression of reaction to *Pst* against number of favorable alleles in 143 wheat accessions. (a) BLUP_IT (b) BLUP_FDS (c) BLUP_AUDPC. Four stable QTL for APR, including *QYrsicau-2B.1*, *QYrsicau-3B.3*, *QYrsicau-5B.2* and *QYrsicau-5B.3*, were selected for analysis

See this image and copyright information in PMC

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Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

Affiliations

Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

Authors

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Abstract

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