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Review
. 2020 Dec;21(8):564-575.
doi: 10.2174/1389202921999201020203249.

Single-cell RNA Sequencing in Immunology

Yirui Cao  1 , Yue Qiu  1 , Guowei Tu  1 , Cheng Yang  1
Affiliations
Review

Single-cell RNA Sequencing in Immunology

Yirui Cao et al. Curr Genomics. 2020 Dec.

Abstract

The complex immune system is involved in multiple pathological processes. Single-cell RNA sequencing (scRNA-seq) is able to analyze complex cell mixtures correct to a single cell and single molecule, thus is qualified to analyze immune reactions in several diseases. In recent years, scRNA-seq has been applied in many researching fields and has presented many innovative results. In this review, we intend to provide an overview of single-cell RNA sequencing applications in immunology and a prospect of future directions.

Keywords: Single-cell RNA sequencing; autoimmune disease; cancer; immunology; transplantation; tumors.

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References

    1. Furman D., Davis M.M. New approaches to understanding the immune response to vaccination and infection. Vaccine. 2015;33(40):5271–5281. doi: 10.1016/j.vaccine.2015.06.117. - DOI - PMC - PubMed
    1. Massaia A., Chaves P., Samari S., Miragaia R.J., Meyer K., Teichmann S.A., Noseda M. Single cell gene expression to understand the dynamic architecture of the heart. Front. Cardiovasc. Med. 2018;5:167. doi: 10.3389/fcvm.2018.00167. - DOI - PMC - PubMed
    1. Chen H., Ye F., Guo G. Revolutionizing immunology with single-cell RNA sequencing. Cell. Mol. Immunol. 2019;16(3):242–249. doi: 10.1038/s41423-019-0214-4. - DOI - PMC - PubMed
    1. Papalexi E., Satija R. Single-cell RNA sequencing to explore immune cell heterogeneity. Nat. Rev. Immunol. 2018;18(1):35–45. doi: 10.1038/nri.2017.76. - DOI - PubMed
    1. Luecken M.D., Theis F.J. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol. Syst. Biol. 2019;15(6):e8746. doi: 10.15252/msb.20188746. - DOI - PMC - PubMed

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