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. 2020 Dec 19:2020:4345804.
doi: 10.1155/2020/4345804. eCollection 2020.

Inhibitory Effect of Lactobacillus plantarum CCFM8724 towards Streptococcus mutans- and Candida albicans-Induced Caries in Rats

Qiuxiang Zhang  1   2   3 , Sujia Qin  1   3 , Xianyin Xu  4 , Jianxin Zhao  1   3 , Hao Zhang  1   3   5   6 , Zhenmin Liu  2 , Wei Chen  1   3   5   7
Affiliations

Inhibitory Effect of Lactobacillus plantarum CCFM8724 towards Streptococcus mutans- and Candida albicans-Induced Caries in Rats

Qiuxiang Zhang et al. Oxid Med Cell Longev. .

Abstract

Streptococcus mutans is a recognized cariogenic bacterium and a major producer of biofilm matrix. The presence of Candida albicans in dental plaque with S. mutans enhances the virulence leading to the onset of rampant caries which is similar to early childhood caries (ECC). The purpose of this study was to explore the effect of Lactobacillus plantarum CCFM8724 (CCFM8724) on the treatment and prevention of dental caries induced by S. mutans and C. albicans in vivo. Rats were divided into 6 groups: the control group and model group, 2 treatment groups, and 2 prevention groups (0.02% chlorhexidine or CCFM8724). The fluctuation of microbial colonization and the change of bacteria flora in rat oral cavity after sowing of L. plantarum CCFM8724 were investigated by colony-forming units (CFU) and microflora analysis. The caries of rats were assessed by microcomputed tomography (micro-CT) and Keyes scoring method. The results showed that L. plantarum CCFM8724 in both the treatment and prevention groups could significantly decrease the population of S. mutans and C. albicans in the rats' oral cavity (p < 0.001), the mineral loss of enamel (p < 0.05), and the scores of caries (p < 0.05). Besides, L. plantarum CCFM8724 exhibited better effects than chlorhexidine. Hence, L. plantarum CCFM8724 was proved to be a potential oral probiotic on caries treatment and prevention in vivo and it may have the prospect of application in dental caries (especially ECC) prevention products.

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Conflict of interest statement

The authors declare no conflict of interests.

Figures

Figure 1
Figure 1
Animal experimental design.
Figure 2
Figure 2
Microorganism count (log CFU/mL) recovered from rat oral swabs for 4 times. (a) Counting results of S. mutans. (b) Counting results of C. albicans. Values are significantly different from the caries model group in each sampling at ∗p < 0.05, ∗∗p < 0.01, or ∗∗∗p < 0.001 (a, b). (c) Counting results of L. plantarum. Groups with dissimilar letters differ, p < 0.05.
Figure 3
Figure 3
Changes in the level of genus in the oral cavity of rats in different groups. (a) Relative abundance of main genus > 0.1% in different groups. (b) Changes of the abundance of Streptococcus genus in different groups. Groups with dissimilar letters differ, p < 0.05. (c) Changes of the abundance of Lactobacillus genus in different groups. Groups with dissimilar letters differ, p < 0.05.
Figure 4
Figure 4
3D micro-CT image of mandibular molars, separated enamel (green), and corresponding 2D scale sagittal slice of the same molar (enamel is green) in each group. Red arrows: caries lesion site.
Figure 5
Figure 5
(a) Volume of enamel of mandibular molars. (b) MD of enamel of mandibular molars. Groups with dissimilar letters differ, p < 0.05.
See this image and copyright information in PMC

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