In vivo evaluation of Nano-palladium toxicity on larval stages and adult of zebrafish (Danio rerio)

Abstract

The existence and usage of nano-sized palladium (nano-Pd) as catalytic promoters among industries and researchers have been laid a way to explore the release of nano-Pd particles into the aquatic environment, bio-accumulating in living organisms. However, the data on fate and toxicity in response to nano-Pd on aquatic organisms are very limited. Herein, we report the concentration-specific toxicity of nano-Pd in zebrafish (Danio rerio). Nano-Pd was synthesized and characterized by Field Emission Scanning Electron Microscopy (FE-SEM), Dynamic Light Scattering (DLS) and Zeta potential. To determine the in vivo toxicity of nano-Pd, the 96 hpf larvae and the adult zebrafish were treated with two (22 and 0.4 ng/L) environmental relevant concentrations. High doses of nano-Pd influenced the hatching rate, embryo survival, heartbeat and teratological anomalies in the 96 hpf larvae. Reactive oxygen species (ROS) and apoptosis were also influenced by nano-Pd exposure while the acetylcholinesterase (AChE) activity was declined in a dose dependent manner. In long-term exposure (42 days), the adult fish showed erratic movements in swimming pattern inhibiting the AChE activity in both the concentrations of brain and liver. The antioxidant enzyme activity such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR) and lipid peroxidation (LPO), showed a significant change (P < 0.05) indicating that oxidative stress was induced by nano-Pd. Similarly, nano-Pd also induced histopathological lesions in gill, liver and brain providing an insight of fate and toxicity of nano-Pd in the aquatic environment. Our study contributes a significant mechanism to understand the toxicity concern of nano-Pd in the aquatic environment.

Keywords: Antioxidant enzymes; Embryonic malformation; Neurotoxicity; Palladium nanoparticle; ROS and apoptosis.