Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jan 1;249(1):297-300.
doi: 10.1042/bj2490297.

Histidine decarboxylase from rat mast cells. Enhanced recovery in cell-free extracts and isotopic labelling

R S Feldberg  1 , D A Iannitti, D E Cochrane
Affiliations

Histidine decarboxylase from rat mast cells. Enhanced recovery in cell-free extracts and isotopic labelling

R S Feldberg et al. Biochem J. .

Abstract

A procedure for obtaining rat mast-cell histidine decarboxylase in greater than 50% yield in cell-free extracts was developed. The enzyme was found in the supernatant fractions from a 3,500 g and a 105,000 g centrifugation step and was demonstrated to be sensitive to inhibition by alpha-fluoromethylhistidine but not by phenylalanine. Although the enzyme shows a half-life of only 3 h in cell-free extract, the initial high recovery of activity allowed for active-site labelling of the enzyme by [3H]histidine and NaBH4. Labelled protein migrated on non-denaturing polyacrylamide-gradient-gel electrophoresis as a 55,000 Da species.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1974 Feb;71(2):408-12 - PubMed
    1. N Engl J Med. 1976 Feb 5;294(6):320-5 - PubMed
    1. Annu Rev Pharmacol Toxicol. 1977;17:325-39 - PubMed
    1. Anal Biochem. 1978 Feb;84(2):638-41 - PubMed
    1. Nature. 1978 Aug 31;274(5674):906-8 - PubMed

Publication types