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. 2021 Jan;28(1):50-54.
doi: 10.1016/j.sjbs.2020.08.030. Epub 2020 Aug 26.

The Role of Reticulocyte Hemoglobin Content for the Diagnosis of Functional Iron Deficiency in Hemodialyzed patients

Ali A Alageeli  1 , Fatmah S Alqahtany  2 , Farjah H Algahtani  3
Affiliations

The Role of Reticulocyte Hemoglobin Content for the Diagnosis of Functional Iron Deficiency in Hemodialyzed patients

Ali A Alageeli et al. Saudi J Biol Sci. 2021 Jan.

Abstract

The effectiveness of reticulocyte hemoglobin content (CHr) had been reported to detect early functional iron deficiency especially among Chronic kidney disease (CKD) patients. CHr is more superior to classic biochemical indices in reflecting transient iron-deficiency status, therefore improving diagnosis and treatment. This study was conducted to determine the sensitivity of CHr in the diagnosis of functional iron deficiency (FID) in hemodialyzed patients. One hundred hemodialyzed patients along with 60 healthy controls were recruited and blood specimens were collected. Venous blood was used for hematological and biochemical investigations collected via 3 ml lavender-top tubes for hematological tests including CBC, blood film, ESR and CHr, and red-top tube for biochemical tests including TIBC, SF and CRP. A statistically significant decrease was noted in CHr values between hemodialysis patients and the control group (24.8 ± 2.0 pg vs. 30.9 ± 1.3 pg, p<0.001). CHr values showed a significant correlations with RBCs, Hb- hemoglobin, Hct- hematocrit level, MCV- mean corpuscular volume, MCH- mean corpuscular hemoglobin, MCHC, RDW- red cell distribution width , SI-Serum Iron, TIBC- Total iron binding capacity and TSAT- Transferrin saturation. The present study showed that CHr in comparison to the conventional hematological and biochemical markers commonly used to diagnose iron deficiency.

Keywords: hemodialysis; hemoglobin; reticulocytes.

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Figures

Figure 1
Figure 1
Correlations between CHr values and haematological parameters in CRF patients.
Figure 2
Figure 2
Li near regression analysis showing the correlations between CHr values and biochemical parameters in CRF patients.
See this image and copyright information in PMC

References

    1. Knutson M.D. Iron transport proteins: Gateways of cellular and systemic iron homeostasis. J Biol Chem. 2017;292(31):12735–12743. - PMC - PubMed
    1. Rostoker G., Vaziri N.D., Fishbane S. Iatrogenic Iron Overload in Dialysis Patients at the Beginning of the 21st Century. Drugs. 2016;76(7):741–757. - PMC - PubMed
    1. Zumbrennen-Bullough K., Babitt J.L. The iron cycle in chronic kidney disease (CKD): from genetics and experimental models to CKD patients. Nephrol Dial Transplant. 2014;29(2):263–273. - PMC - PubMed
    1. Kuo K.L. Association of Anemia and Iron Parameters With Mortality Among Patients Undergoing Prevalent Hemodialysis in Taiwan: The AIM - HD Study. J Am Heart Assoc. 2018;7(15) - PMC - PubMed
    1. Chavers B.M. Prevalence of anemia in erythropoietin-treated pediatric as compared to adult chronic dialysis patients. Kidney Int. 2004;65(1):266–273. - PubMed