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. 2021 Jan;28(1):247-254.
doi: 10.1016/j.sjbs.2020.09.056. Epub 2020 Oct 8.

Quantification of biochemical compounds in Bauhinia Variegata Linn flower extract and its hepatoprotective effect

Hina Gul  1 , Muhammad Awais  1   2 , Salina Saddick  3 , Yazeed Ahmed  4 , Falak Sher Khan  5 , Eilaf Ahmed  4 , Umara Afzal  1   6 , Syed Muhammad Zaigham Abbas Naqvi  7 , Muhammad Asghar Khan  8 , Muhammad Gulfraz  1 , Ghazala Kaukab Raja  1
Affiliations

Quantification of biochemical compounds in Bauhinia Variegata Linn flower extract and its hepatoprotective effect

Hina Gul et al. Saudi J Biol Sci. 2021 Jan.

Abstract

Liver disorders may occur as a result of exposure to chemical compounds capable of inducing the oxidative stress and hepatic injuries. The aim of present study was to investigate the effects of flower extracts of B. Variegata for the treatment of liver injury induced by the CCl4. About 1 ml/kg body weight (b.w) of CCl4 was induced to experimental mice by intraperitoneal way for 14 days. The methanol and chloroform extracts (100, 200 and 300 mg/kg b.w) were administered to experimental animals for 14 days along with standard drug Silymarine (100 mg/kg b.w). The extracts alone showed no evidence of hepatic toxicity but animals exposed to CCl4 without the treatment with B. Variegata presented variations in levels of liver enzymes, antioxidant enzymes, proteins and blood cells as well as injuries in liver cells were also observed during histopathological study. However, after the treatments especially with 300 mg/kg b.w of methanol flower extracts levels of liver markers (ALT, AST and ALP), antioxidant enzymes and blood cells decreases and turned towards normal levels. Whereas level of total proteins and bilirubin was improved and damaged liver cells were repaired. The curative activity of flower extracts can be correlated to the higher potential of antioxidants and occurrence of Quercetin and some other organic compounds those were investigated from flower extracts of B. Variegata during HPLC and GC-MS analysis. The finding of this study supports the use of B. Variegata flower formulation in folk medicines.

Keywords: Antioxidants; B. Variegata; HPLC; Hepatoprotective effect; Phytochemicals.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Chromatogram of Quercetin standard.
Fig. 2
Fig. 2
HPLC analysis of Quercetin from B. variegata.
Fig. 3
Fig. 3
Chromatogram showing analysis of methanol flower extracts of B. Variegata by using GC–MS.
Fig. 4
Fig. 4
Comparison of initial and final body weight of mice.
Fig. 5
Fig. 5
Response of the animal group against different doses were recorded n = 5.Whereas RBC, WBC and Platelets count is measured in 106 /µl, 103 /µl and 103 /µl.
Fig. 6
Fig. 6
a: CCl4 induced toxicity in the liver by changing its symmetry. b:Damaged liver treated with B.V flowers tried to cure the injury.
See this image and copyright information in PMC

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