Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec 24;5(4):3819-3820.
doi: 10.1080/23802359.2020.1840935.

The complete chloroplast genome of Stephania tetrandra (Menispermaceae)

Affiliations

The complete chloroplast genome of Stephania tetrandra (Menispermaceae)

Lan Cao et al. Mitochondrial DNA B Resour. .

Abstract

The complete chloroplast genome of Stephania tetrandra was sequenced and assembled for the first time. The chloroplast genome is 159,974 bp in length, containing a large single-copy (LSC) region of 90,539 bp and a small single-copy region (SSC) of 20,735 bp, separated by a pair of inverted repeats (IRs) of 24,350 bp. The genome contains 113 unique genes, including 79 protein-coding genes (PCGs), 30 tRNA genes, and four rRNA genes. Among them, 15 genes have one intron each and three genes contain two introns. The overall GC content is 37.8%, while the corresponding values of LSC, SSC, and IR regions are 35.8, 32.4, and 43.7%, respectively. Phylogenetic analysis showed that S. tetrandra is more closely related to the clade of two species within Stephania, providing new insight into the evolution of Menispermaceae.

Keywords: Complete chloroplast genome; Menispermaceae; Stephania tetrandra.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Maximum-likelihood phylogenetic tree based on complete cp genomes. Numbers close to each node are bootstrap support values.

References

    1. Doyle JJ, Doyle JL.. 1987. A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochem Bull. 19:11–15.
    1. Jin JJ, Yu WB, Yang JB, Song Y ,Yi TS ,Li DZ.. 2020. GetOrganelle: a simple and fast pipeline for de novo assembly of a complete circular chloroplast genome using genome skimming data. Genome biology 21(1): 1–31. - PMC - PubMed
    1. Katoh K, Misawa K, Kuma KI, Miyata T.. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 30(14):3059–3066. - PMC - PubMed
    1. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, et al. . 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 28(12):1647–1649. - PMC - PubMed
    1. Lowe TM, Chan PP.. 2016. tRNAscan-SE on-line: integrating search and context for analysis of transfer RNA genes. Nucleic Acids Res. 44(W1):W54–W57. - PMC - PubMed

LinkOut - more resources