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Comparative Study
. 2021 Jan 11;21(1):25.
doi: 10.1186/s12866-020-02073-2.

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among neonates

Chun-Mei Chen  1 , Min Wang  2 , Xian-Ping Li  1 , Peng-Ling Li  3 , Jing-Jing Tian  1 , Kan Zhang  1 , Can Luo  1
Affiliations
Comparative Study

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among neonates

Chun-Mei Chen et al. BMC Microbiol. .

Erratum in

Abstract

Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. However, the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn't been characterized yet.

Results: Forty-three isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes, and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. The results of MLST were consist with the results of PFGE.

Conclusions: These data showed that there might be a close homologous relationship between extraintestinal K. pneumoniae (EXKP) and enteral K. pneumoniae (EKP) in neonates, indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

Keywords: Antibiotic resistance; Endogenic infection; Gastrointestinal colonization; Klebsiella pneumoniae; Multiple locus sequence typing.

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Conflict of interest statement

The funding bodies played the role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The UMPGA dendrogram, sequence types (STs), and genotypes of 43 Klebsiella pneumoniae isolates from 21 patients. The tree shows that ST37 and ST1083 were related. In our previous study, ST37 and ST1083 belong to the same clone complex CC37 [19]
Fig. 2
Fig. 2
Dendrogram comparing PFGE profile of K. pneumoniae (patients 12, 15 and 21) together with the results of MLST. The PFGE shows that the paired isolates from patients 12, 15 and 21 had identical and > 90% similarity in PFGE patterns
See this image and copyright information in PMC

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References

    1. Conlan S, Kong HH, Segre JA. Species-level analysis of DNA sequence data from the NIH human microbiome project. PLoS One. 2012;7:e47075. doi: 10.1371/journal.pone.0047075. - DOI - PMC - PubMed
    1. Farida H, Severin JA, Gasem MH, et al. Nasopharyngeal carriage of Klebsiella pneumoniae and other gram-negative bacilli in pneumonia-prone age groups in Semarang, Indonesia. J Clin Microbiol. 2013;51:1614–1616. doi: 10.1128/JCM.00589-13. - DOI - PMC - PubMed
    1. Dao TT, Liebenthal D, Tran TK, et al. Klebsiella pneumoniae oropharyngeal carriage in rural and urban Vietnam and the effect of alcohol consumption. PLoS One. 2014;9:e91999. doi: 10.1371/journal.pone.0091999. - DOI - PMC - PubMed
    1. Dorman MJ, Short FL. Genome watch: Klebsiella pneumoniae: when a colonizer turns bad. Nat Rev Microbiol. 2017;15:384. doi: 10.1038/nrmicro.2017.64. - DOI - PubMed
    1. Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998;11:589–603. doi: 10.1128/CMR.11.4.589. - DOI - PMC - PubMed

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