Effects of elevated CO2 on arbuscular mycorrhizal fungi associated with Robinia pseudoacacia L. grown in cadmium-contaminated soils

Abstract

As symbionts capable of reciprocal rewards, arbuscular mycorrhizal fungi (AMF) can alleviate heavy metal toxicity to host plants and are easily influenced by elevated CO₂ (ECO₂). Although the individual effects of ECO₂ and cadmium (Cd) on AMF have been widely reported, the response of AMF to ECO₂ + Cd receives little attention. We evaluated the combined effects of ECO₂ and Cd on AMF in the rhizosphere soil and roots of Robinia pseudoacacia L. seedlings. Under ECO₂ + Cd relative to Cd, AMF gene copies and richness in rhizosphere soils increased (p < 0.05) and the diversity reduced (p < 0.05) at 4.5 mg Cd kg^-1 dry soil; whereas root AMF abundance at 4.5 mg Cd kg^-1 dry soil and the diversity and richness reduced (p < 0.05). Elevated CO₂ caused obvious differences in the dominant genera abundance between rhizosphere soils and roots upon Cd exposure. Responses of C, water-soluble organic nitrogen (WSON), pH, and diethylene triamine penta-acetic acid (DTPA)-Cd in rhizosphere soils and root N to ECO₂ shaped dominant genera in Cd-polluted rhizosphere soils. Levels of DTPA-Cd, WSON, C and pH in rhizosphere soils and C/N ratio, N, and Cd in roots to ECO₂ affected (p < 0.05) dominant genera in roots under Cd exposure. AMF richness and diversity were lower in roots than in rhizosphere soils. Elevated CO₂ altered AMF communities in rhizosphere soils and roots of R. pseudoacacia seedlings exposed to Cd. AMF associated with R. pseudoacacia may be useful/interesting to be used for improving the phytoremediation of Cd under ECO₂.

Keywords: Abundance; Arbuscular mycorrhizal fungi; Cadmium-contaminated soils; Community structure; Elevated CO(2); Phytoremediation.