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. 2021 Jan 12;11(1):650.
doi: 10.1038/s41598-020-80133-3.

Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Reza Eivazzadeh-Keihan  1 , Fateme Radinekiyan  1 , Hooman Aghamirza Moghim Aliabadi  2   3 , Sima Sukhtezari  1 , Behnam Tahmasebi  4 , Ali Maleki  5 , Hamid Madanchi  6   7
Affiliations

Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Reza Eivazzadeh-Keihan et al. Sci Rep. .

Retraction in

Abstract

Herein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Synthesis preparation of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold.
Figure 2
Figure 2
FT-IR spectra of (a) chitosan biopolymer, (b) CTT-CS hydrogel, (c) CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold.
Figure 3
Figure 3
(a) EDX spectrum, (b) elemental mapping images of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold.
Figure 4
Figure 4
FE-SEM images of (a,b) CTT-CS hydrogel, (c) CTT-CS/SF hydrogel, (d) synthetic CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold (freeze-dried structures).
Figure 5
Figure 5
Thermogravimetric curve of synthetic CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold.
Figure 6
Figure 6
Compressive strength of the scaffolds from the four groups. Data represent the mean ± SD (n = 3).
Figure 7
Figure 7
(a) Cell viability histogram of untreated Hu02 cells as control, CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold and cisplatin treatments at 3 days and 7 days, (b) image of microplate well from MTT assay on Hu02 cell line, (c) inverted microscope images of untreated cells as control, CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold and cisplatin treatments.
Figure 8
Figure 8
(a,b) Hemolysis histogram and 96-well plate image of Triton X-100 (positive control), CTT-CS hydrogel, CTT-CS/SF hydrogel, CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, and 0.9% NaCl (negative control).
Figure 9
Figure 9
(a,b) Anti-biofilm histogram and 96-well plate image of polystyrene piece, CTT-CS hydrogel, CTT-CS/SF/Mg(OH)2 nanobiocomposite. It was clear that synthesized nanobiocomposite scaffold was able to inhibit the P. aeruginosa biofilm formation.
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