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. 2021 Jan 14;10(2):e01017-20.
doi: 10.1128/MRA.01017-20.

Draft Genome Sequence of Pseudomonas sp. Strain FEN, Isolated from the Fe- and Organic Matter-Rich Schlöppnerbrunnen Fen

Rebecca E Cooper  1 , Carl-Eric Wegner  2 , Kirsten Küsel  2   3
Affiliations

Draft Genome Sequence of Pseudomonas sp. Strain FEN, Isolated from the Fe- and Organic Matter-Rich Schlöppnerbrunnen Fen

Rebecca E Cooper et al. Microbiol Resour Announc. .

Abstract

Here, we report the draft genome sequence of Pseudomonas sp. strain FEN, a nonfluorescent siderophore producer that was isolated from the Schlöppnerbrunnen fen, which is characterized by high concentrations of Fe, dissolved organic matter (DOM), and Fe-DOM complexes. This draft genome sequence provides insight into the mechanisms of siderophore biosynthesis and siderophore-mediated iron uptake by this bacterium.

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Figures

Fig 1
Fig 1
Phylogenetic analysis based on pairwise comparisons of Pseudomonas sp. FEN versus type strain genomes and ANI-based analysis of publicly available genome sequences of siderophore-producing Pseudomonas sp. strains. (A) Phylogenetic tree based on whole-genome comparisons using the TYGS. The phylogenetic tree was inferred using FASTME v2.1.6.1 from genome-based distance phylogeny (GBDP) distances calculated from closely related genome sequences. The branch lengths are scaled based on GBDP distances. The numbers above each branch represent GBDP pseudo-bootstrap values of >60% from 100 replications. The tree was rooted at the midpoint. (B) ANI genome-based distance matrix calculator output, based on pairwise ANI between different siderophore-producing Pseudomonas sp. strains within the phylum Proteobacteria with publicly available genomes, including the Pseudomonas sp. FEN isolate described here. All ANI values are shown in the matrix. Pseudomonas cepacia was renamed Burkholderia cepacia ATCC 25416 and was used in the ANI-based analysis due to its siderophore-producing phenotype.
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