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. 2021 Dec;13(1_suppl):797S-808S.
doi: 10.1177/1947603520988165. Epub 2021 Jan 16.

Patients with Osteoarthritis and Kashin-Beck Disease Display Distinct CpG Methylation Profiles in the DIO2, GPX3, and TXRND1 Promoter Regions

Rongqiang Zhang  1   2 , Hao Guo  2 , Xiaoli Yang  2 , Dandan Zhang  2 , Di Zhang  2 , Qiang Li  2 , Chen Wang  2 , Xuena Yang  2 , Yongmin Xiong  2
Affiliations

Patients with Osteoarthritis and Kashin-Beck Disease Display Distinct CpG Methylation Profiles in the DIO2, GPX3, and TXRND1 Promoter Regions

Rongqiang Zhang et al. Cartilage. 2021 Dec.

Abstract

Objective: We aimed to analyze deoxycytidine-deoxyguanosine dinucleotide (CpGs) methylation profiles in DIO2, GPX3, and TXNRD1 promoter regions in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients.

Methods: Blood samples were collected from 16 primary OA patients and corresponding 16 healthy individuals and analyzed for methylations in the CpGs of DIO2, GPX3, and TXNRD1 promoter regions using MALDI-TOF-MS. The methylation profiles of these regions were then compared between OA and KBD patients.

Results: DIO2-1_CpG_2 and DIO2-1_CpG_3 methylations were significantly lower in OA than KBD patients (P < 0.05). A similar trend was observed for GPX3-1_CpG_4, GPX3-1_CpG_7, GPX3-1_CpG_8.9.10, GPX3-1_CpG_13.14.15 and GPX3-1_CpG_16 (P < 0.05) as well as TXNRD1-1_CpG_1 and TXNRD1-1_CpG_2 methylation between OA and KBD patients (P < 0.05). However, there was no difference in methylation levels of other CpGs between the 2 groups (P > 0.05).

Conclusion: OA and KBD patients display distinct methylation profiles in the CpG sites of DIO2, GPX3, and TXNRD1 promoter regions. These findings provide a strong background and new perspective for future studies on mechanisms underlying epigenetic regulation of selenoprotein genes associated with OA and KBD diseases.

Keywords: CpG; CpG-SNP; DNA methylation; Kashin-Beck disease; osteoarthritis.

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Conflict of interest statement

Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Methylation levels of CpGs in the promoter region of DIO2 gene in patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The color intensity is directly proportional to the methylation level. The numbers 1, 2, 3, and 4 represent CpGs DIO2-1_CpG_1, DIO2-1_CpG_2, DIO2-1_CpG_3, and DIO2-1_CpG_4, respectively.
Figure 2.
Figure 2.
Methylation levels of CpG sites in DIO2 promoter region in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients.
Figure 3.
Figure 3.
Methylation levels of CpGs in the promoter region of GPX3 gene in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients. The color intensity of the dots is directly proportional to the methylation level. The numbers 1 to 25 in the figure represent CpGs GPX3-8_CpG_1 - GPX3-8_CpG_25.
Figure 4.
Figure 4.
Methylation levels of CpGs in the promoter region of GPX3 in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients.
Figure 5.
Figure 5.
Methylation levels of CpGs in the promoter region of the TXNRD1 gene in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients. The color intensity of the dots is directly proportional to the methylation level. The numbers 1 to 13 represent CpGs TXNRD1-38_CpG_1- to TXNRD1-38_CpG_13.
Figure 6.
Figure 6.
Methylation levels of CpGs in the promoter region of TXNRD1 in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients.
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