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. 2020 May-Aug;24(2):399.
doi: 10.4103/jomfp.JOMFP_289_19. Epub 2020 Sep 9.

Proliferative and anti-apoptotic indices of unicystic ameloblastoma, odontogenic keratocyst, dentigerous cyst and radicular cyst

Efetobo Victor Orikpete  1 , Osawe Felix Omoregie  2 , Michael Akin Ojo  2
Affiliations

Proliferative and anti-apoptotic indices of unicystic ameloblastoma, odontogenic keratocyst, dentigerous cyst and radicular cyst

Efetobo Victor Orikpete et al. J Oral Maxillofac Pathol. 2020 May-Aug.

Abstract

Objective: The aim of this study is to compare the proliferative capacity and antiapoptotic capacity of unicystic ameloblastoma (UA), odontogenic keratocyst (OKC), dentigerous cyst (DC) and radicular cyst (RC) by assessing the Ki-67 labeling index (LI) and Bcl-2 LI, respectively.

Materials and methods: Formalin-fixed, paraffin-embedded tissue blocks of twenty-three histopathologically diagnosed UAs, 6 OKCs, 8 DCs and 10 RCs were selected from the archival specimens. Five micrometer thick sections of tissue blocks of the lesions were processed for immunohistochemical staining with Ki-67 and Bcl-2. The Ki-67 LI and Bcl-2 LI were determined for the cases with a positive reaction.

Results: Ki-67 expression was seen in 6 (26.1%) cases of UA, 4 (66.7%) OKC, 1 (12.5%) DC and 1 (10.0%) RC. There was a statistically significant difference between the mean Ki-67 LI of UA and OKC (P = 0.024). Bcl-2 expression was seen in 16 (69.6%) UA, 5 (83.3%) OKC, 5 (62.5%) DC and 5 (50.0%) RC. The mean Bcl-2 LI of UA was significantly higher than that of DC (P = 0.048). Furthermore, cases of OKC had significantly higher mean Bcl-2 LI compared to DC (P = 0.026) and RC (P = 0.049).

Conclusion: This study suggests that the Ki-67 LI may help in differentiating OKC from UA. The Bcl-2 LI may be useful in differentiating UA from DCs, and differentiating OKC from DC and RC.

Keywords: Bcl-2; Ki-67; odontogenic cysts; unicystic ameloblastoma.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
(a) Photomicrograph showing focal Ki-67 immunoreaction in the basal layer of unicystic ameloblastoma (arrows) (×400). (b) Photomicrograph showing positive Ki-67 immunoexpression in OKC mainly in the suprabasal cell layer (arrows) (×400). (c) Photomicrograph showing mild, focal expression of Ki-67 in dentigerous cyst epithelium (arrow) (×400). (d) Photomicrograph showing positive immunoexpression of Ki-67 in radicular cyst epithelium. (×400)
Figure 2
Figure 2
(a) Photomicrograph showing strong positive Bcl-2 immunoreaction in the ameloblast-like cells of unicystic ameloblastoma (×100). (b) Photomicrograph showing positive Bcl-2 immunoexpression in OKC mainly in the basal cell layer (arrows) (×400). (c) Photomicrograph of dentigerous cyst showing positive immunoexpression of Bcl-2. (arrows) (×400). (d) Photomicrograph showing positive immunoexpression of Bcl-2 in radicular cyst epithelium (black arrows). Note also the positively stained cells in the connective tissue capsule interpreted to be lymphocytes (red arrows) (× 400)
Figure 3
Figure 3
Error bars comparing the mean Bcl-2 labelling indices
See this image and copyright information in PMC

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