Expression and significance of S-100β, CysC and NF-κB in patients with acute cerebral infarction

Abstract

The present study aimed to explore the expression and significance of S100 protein β (S100β), cystatin C (CysC), and nuclear factor kappa B (NF-κB) in patients with acute cerebral infarction (ACI). ACI patients (n=120) were selected as the experimental group at Xuzhou Central Hospital from August 2016 to August 2018. Ninety healthy subjects who underwent a physical examination at Xuzhou Central Hospital during the same period were selected as the control group. The expression levels of S-100β, CysC and NF-κB were compared between the two groups. Serum S-100β, CysC and NF-κB levels were compared between ACI patients with different degree of nervous functional defects, different infarct size and different prognosis. ROC curve analysis was used for the diagnosis of ACI by serum S-100β, CysC and NF-κB levels. Serum S-100β, CysC and NF-κB levels in the experimental group were higher than those in the control group (P<0.05). The levels of serum S-100β, CysC and NF-κB in patients with different neurological deficits were significantly different. The levels of serum S-100β, CysC and NF-κB in the severe and medium type infarction group were significantly higher than those in the mild type infarction group (both P<0.05). The levels of serum S-100β, CysC and NF-κB in the severe type infarction group were higher than those in the medium type infarction group (P<0.05). There were significant differences in serum S-100β, CysC and NF-κB levels in patients with different infarct sizes. The levels of serum S-100β, CysC and NF-κB in patients with large and medium size infarction were higher than those in the small size infarction group (both P<0.05). The levels of serum S-100β, CysC and NF-κB in patients with large size infarction were higher than those in patients with medium size infarction (P<0.05). Serum S-100β, CysC and NF-κB levels in patients of the worsening group were significantly higher than those in patients of the non-worsening group. The levels of S-100β, CysC, NF-κB in ACI patients were significantly higher than those in healthy subjects. Increased levels of S-100β, CysC and NF-κB can be used as ideal indexes for diagnosing cerebral infarction and studying the condition.

Keywords: S100 protein β; acute cerebral infarction; cystatin C; diagnose; nuclear factor κB.

Figure 1

ROC curve of S-100β, CysC and NF-κB levels for diagnosis value of ACI. (A) The diagnostic sensitivity of S-100β to ACI was 67.78%. The specificity was 90.08%. The AUC was 0.845. The 95% CI was 0.791-0.899. The critical value was 0.985 ρ/µg·l^-1. (B) The diagnostic sensitivity of CysC to ACI was 92.22%. The specificity was 70.00%. The AUC was 0.823. The 95% CI was 0.765-0.879. The critical value was 1.193 mg/l. (C) The diagnostic sensitivity of NF-κB to ACI was 83.33%. The specificity was 57.50%. The AUC was 0.745. The 95% CI was 0.679-0.810. The critical value was 317.7 pg/ml. ROC, receiver operating characteristic; S100β, S100 protein β; CysC, cystatin C; NF-κB, nuclear factor kappa B; ACI, acute cerebral infarction;