The complete chloroplast genomes of two Mexican plants of the annual herb Datura stramonium (Solanaceae)

Abstract

The annual herb, Datura stramonium, is a member of the Solanaceae family. In this study, we report the chloroplast genomes of two Mexican plants of D. stramonium. Both chloroplast genomes of D. stramonium (GenBank accessions: MT610896 and MT610897) were assembled as a circular molecule. The genome size of both plants was similar (155,884 bp). The overall GC content was 38.59% for both genomes. Both chloroplast genomes contained 85 protein-coding sequences (CDS), 131 genes, 8 rRNA genes, and 38 tRNA genes. Thirty-nine microsatellites (SSRs) and 42 long tandem repeats were also identified for both genomes. The phylogenetic relationship between D. stramonium and related Solanaceae species revealed four main groups; Nicotiana, Datura, Capsicum, and Solanum clades. This species tree is consistent with other Solanaceae species trees already published.

Keywords: Chloroplast assembly; Datura stramonium; Organelle; Solanaceae.

Figure 1.

ML phylogenetic tree of D. stramonium with 13 species was constructed by chloroplast sequence. Numbers in the nodes are bootstrap values from 1000 replicates. Arabidopsis thaliana was set as outgroup. NCBI accession of each chloroplast genome is indicated within the parenthesis.