doi: 10.1016/0014-4827(88)90254-6.
Immunocytochemical localization of glucocorticoid receptors in cells, cytoplasts, and nucleoplasts
Affiliations
- PMID: 3345802
- DOI: 10.1016/0014-4827(88)90254-6
Item in Clipboard
Immunocytochemical localization of glucocorticoid receptors in cells, cytoplasts, and nucleoplasts
Exp Cell Res.
1988 Mar.
Abstract
A monoclonal antibody has been used to assess the intracellular localization of the glucocorticoid receptor in rodent L-929 fibroblasts and GH3 pituitary tumor cells. Whole cells from both cell lines showed immunoreactivity in the cytoplasm and nucleus. However, when cytoplasts and nucleoplasts of these cells were examined, only L-cells showed strong antibody binding in both fractions; in contrast, GH3 cells exhibited nuclear staining and slight cytoplasmic staining. These results are discussed in terms of the current findings regarding the intracellular location of steroid hormone receptors.
Similar articles
-
Nuclear localization of unoccupied receptors for glucocorticoids, estrogens, and progesterone in GH3 cells.Endocrinology. 1985 Nov;117(5):2140-7. doi: 10.1210/endo-117-5-2140. Endocrinology. 1985. PMID: 4042978
-
Intracellular location of unoccupied 1,25-dihydroxyvitamin D receptors: a nuclear-cytoplasmic equilibrium.Arch Biochem Biophys. 1986 Apr;246(1):366-73. doi: 10.1016/0003-9861(86)90481-9. Arch Biochem Biophys. 1986. PMID: 3008657
-
Subcellular compartmentalization of MCF-7 estrogen receptor synthesis and degradation.Mol Cell Endocrinol. 1993 Aug;94(2):183-94. doi: 10.1016/0303-7207(93)90167-i. Mol Cell Endocrinol. 1993. PMID: 8224522
-
Subcellular distribution of the glucocorticoid receptor and evidence for its association with microtubules.J Steroid Biochem Mol Biol. 1995 Jan;52(1):1-16. doi: 10.1016/0960-0760(94)00155-f. J Steroid Biochem Mol Biol. 1995. PMID: 7857868 Review.
-
Intracellular localization of corticosteroid receptors in brain: potential interactions with signal transduction pathways.Proc Soc Exp Biol Med. 1992 Dec;201(3):244-53. doi: 10.3181/00379727-201-43503b. Proc Soc Exp Biol Med. 1992. PMID: 1438340 Review. No abstract available.
Cited by
-
Relationship between expression of epidermal growth factor and simian virus 40 T antigen in a line of transgenic mice.Transgenic Res. 1995 Sep;4(5):306-14. doi: 10.1007/BF01972527. Transgenic Res. 1995. PMID: 8589733
-
Role of luteal cell nucleus in the expression of gonadotropin action.J Endocrinol Invest. 1991 May;14(5):391-400. doi: 10.1007/BF03349088. J Endocrinol Invest. 1991. PMID: 1651965
-
Cell cycle regulation of glucocorticoid receptor function.EMBO J. 1992 Sep;11(9):3457-68. doi: 10.1002/j.1460-2075.1992.tb05425.x. EMBO J. 1992. PMID: 1505524 Free PMC article.
-
Nuclear vs translocating steroid receptor models and the excluded middle.Endocrine. 1995 Jan;3(1):1-4. doi: 10.1007/BF02917441. Endocrine. 1995. PMID: 21153229 No abstract available.
-
Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone.Proc Natl Acad Sci U S A. 1990 Feb;87(3):1086-8. doi: 10.1073/pnas.87.3.1086. Proc Natl Acad Sci U S A. 1990. PMID: 2153968 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
