SMRT-based mitochondrial genome of the edible mushroom Morchella conica

Abstract

Morchella conica Pers. is a highly-prized mushroom for its edible and medical values. In this study, we determined the complete mitochondrial genome of M. conica combining both PacBio and Illumina sequencing technologies. The complete mitochondrial genome is 280,763 bp in length with a GC content of 39.88%. We identified a total of 14 core conserved protein-coding genes, 127 non-conserved open reading frames (ncORFs) and 30 tRNA genes in the M. conica mitogenome. However, no large or small rRNA subunits (rnl or rns) were identified in this mitogenome. In addition, we detected two mitochondrial RNase P (rnpB) genes and one ribosomal protein genes (rps3). Phylogenetic analysis was performed among M. conica and 18 other representative fungi from Ascomycota, Basidiomycota and Mucoromycota. Our results showed that M. conica was most closely related to M. importuna. The availability of the M. conica mitochondrial genome will form the basis of genetic breeding program and enhance our understanding of the evolution of this species.

Keywords: Morchella conica; PacBio; evolution; mitochondrial genome; mushroom.

Figure 1.

Maximum-likelihood (ML) phylogenetic tree of M. conica and related fungal species. The ML-tree is based on 14 conserved core mitochondrial proteins. Bootstraps values (1,000 replicates) are shown at the nodes. All the sequences are currently available in the GenBank database: Botrytis cinerea (KC832409), Epidermophyton floccosum (NC_007394), Fusarium oxysporum (NC_017930), F. verticillioides (NC_016687), Lentinula edodes (NC_018365), M. importuna (NC_045397), Zymoseptoria tritici (NC_010222), Penicillium solitum (NC_016187), Phaeosphaeria nodorum (NC_009746), Phlebia radiata (NC_020148), Pyronema omphalodes (NC_029745), Rhizoctonia solani (NC_021436), Rhynchosporium agropyri (NC_023125), Sclerotinia borealis (NC_025200), Trametes cingulata (NC_013933), Verticillium dahliae (NC_008248) and V. nonalfalfae (NC_029238). Rhizopus oryzae (NC_006836) was served as an outgroup.