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. 2020 Dec 30:25:100892.
doi: 10.1016/j.bbrep.2020.100892. eCollection 2021 Mar.

Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis

Kentaro Tominaga  1 , Atsunori Tsuchiya  1 , Takeshi Mizusawa  1 , Asami Matsumoto  2 , Ayaka Minemura  2 , Kentaro Oka  2 , Motomichi Takahashi  2 , Tomoaki Yosida  1 , Yuzo Kawata  1 , Kazuya Takahashi  1 , Hiroki Sato  1 , Satoshi Ikarashi  1 , Kazunao Hayashi  1 , Ken-Ichi Mizuno  1 , Yosuke Tajima  3 , Masato Nakano  3 , Yoshifumi Shimada  3 , Hitoshi Kameyama  3 , Junji Yokoyama  1 , Toshifumi Wakai  3 , Shuji Terai  1
Affiliations

Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis

Kentaro Tominaga et al. Biochem Biophys Rep. .

Abstract

It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis.

Keywords: AA, acetic acid; BA, butyric acid; DC, diversion colitis; Diversion colitis; IgA, immunoglobulin A; Immunoglobulin A; Microbiota; OA, organic acid; PA, propionic acid; SCFAs, short-chain fatty acids; Short-chain fatty acids.

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Conflict of interest statement

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The authors disclose the following: Shuji Terai received research funding from 10.13039/100016567Miyarisan Pharmaceutical Co., Ltd. Asami Matsumoto, Ayaka Minemura, Kentaro Oka and Motomichi Takahashi are employees of Miyarisan Pharmaceutical Co., Ltd.The remaining authors disclose no conflicts. Please note that all Biochemical and Biophysical Research Communications authors are required to report the following potential conflicts of interest with each submission. If applicable to your manuscript, please provide the necessary declaration in the box above.(1)All third-party financial support for the work in the submitted manuscript.(2)All financial relationships with any entities that could be viewed as relevant to the general area of the submitted manuscript.(3)All sources of revenue with relevance to the submitted work who made payments to you, or to your institution on your behalf, in the 36 months prior to submission.(4)Any other interactions with the sponsor of outside of the submitted work should also be reported. (5) Any relevant patents or copyrights (planned, pending, or issued).(6)Any other relationships or affiliations that may be perceived by readers to have influenced, or give the appearance of potentially influencing, what you wrote in the submitted work. As a general guideline, it is usually better to disclose a relationship than not.

Figures

Fig. 1
Fig. 1
(a) Theα-diversity data of this study. Bars show the SD of the data. Data were analyzed using the Mann–Whitney U test (vs. Pre). (b) The β -diversity data of this study. Bars show the SD of the data. Data were analyzed using the Mann–Whitney U test (vs. Pre) followed by Benjamini–Hochberg (*: P < 0.05).
Fig. 2
Fig. 2
(a) The analysis of the intestinal microbiota. The comparison of microbiota between the diverted colon and the oral colon of the patients, who underwent colostomy. (b) Significant differences were detected in the numbers of Lactobacillus (at genus level) (P < 0.05). Bars show the SD of the data. Data were analyzed using the Mann–Whitney U test (vs. Pre) followed by the Benjamini–Hochberg procedure (*P < 0.05, **p < 0.01).
Fig. 3
Fig. 3
(a) The comparison of intestinal elements between the diverted colon and oral colon. Significant differences were detected for OA (P < 0.05), SCFAs (P < 0.05), AA (P < 0.01), and IgA (P < 0.05). OA; organic acid, SCFA; short-chain fatty acids, AA; acetic acid, BA; butyric acid, PA; propionic acid, IgA; immunoglobulin A. (b) Schematic of diversion colitis.
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