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Review
. 2021 Feb;20(4):345-352.
doi: 10.1080/15384101.2021.1875671. Epub 2021 Jan 18.

Role of Aurora B and Haspin kinases in the metaphase Topoisomerase II checkpoint

M Johansson  1 , Y Azuma  2 , D J Clarke  1
Affiliations
Review

Role of Aurora B and Haspin kinases in the metaphase Topoisomerase II checkpoint

M Johansson et al. Cell Cycle. 2021 Feb.

Abstract

DNA Topoisomerase II (TopoII) uses ATP hydrolysis to decatenate chromosomes so that sister chromatids can faithfully segregate in mitosis. When the TopoII enzyme cycle stalls due to failed ATP hydrolysis, the onset of anaphase is delayed, presumably to allow extra time for decatenation to be completed. Recent evidence revealed that, unlike the spindle assembly checkpoint, this TopoII checkpoint response requires Aurora B and Haspin kinases and is triggered by SUMOylation of the C-terminal domain of TopoII.

Keywords: Aurora B; Haspin; Topoisomerase II; checkpoint; mitosis.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Normal cycling of strand passage reaction. Purple – ATPase Domain, Yellow/Orange – Binding/Cleaving Domain
Figure 2.
Figure 2.
SUMOylated Top2-CTD interacts with Haspin, a kinase that phosphorylates hH3T3. This phosphomark, in turn, provides a binding site for Survivin, a component of the chromosome passenger complex, of which Ipl1/Aurora B is a member
Figure 3.
Figure 3.
Kinetochore Proximal Centromere (KPC). Zone located between the inner centromere and the kinetochore proper
See this image and copyright information in PMC

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