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. 2021 Jan;16(2):71-81.
doi: 10.2217/fmb-2020-0080. Epub 2021 Jan 18.

Evaluation of the antifungal activity in vitro of midazolam against fluconazole-resistant Candida spp. isolates

Maria Av Holanda  1   2 , Cecília R da Silva  1   2 , João B de A Neto  1   2   3 , Lívia G do Av Sá  1   2 , Francisca Bsa do Nascimento  1   2 , Daiana D Barroso  1   2 , Lisandra J da Silva  1   2 , Thiago M Cândido  1   2   3 , Amanda C Leitão  1   2 , Amanda D Barbosa  1   2 , Manoel O de Moraes  2 , Bruno Cc  2 , Hélio V Nobre Júnior  1   2
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Evaluation of the antifungal activity in vitro of midazolam against fluconazole-resistant Candida spp. isolates

Maria Av Holanda et al. Future Microbiol. 2021 Jan.

Abstract

Aim: The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical Candida spp. in planktonic and biofilm form. Materials & methods: The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests were performed to investigate the probable mechanism of action and the comet test and cytotoxicity test were applied to evaluate DNA damage. Results: Midazolam (MIDAZ) showed antifungal activity against planktonic cells (125-250 μg/ml) and reduced the viability of Candida spp. biofilms (125 a 2500 μg/ml). The interaction of MIDAZ against Candida spp. biofilms was observed through scanning electron microscopy, causing alteration of their appearance. Therefore, MIDAZ has antifungal potential against Candida spp.

Keywords: Candida spp.; antifungal potential; biofilm; drug repositioning; midazolam.

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