Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul;84(7):1506-1512.
doi: 10.1002/jemt.23706. Epub 2021 Jan 18.

Biocompatibility of the 8.25% sodium hypochlorite irrigant solution in endodontics: An in vivo study

Luciana S Demenech  1 , Flávia S F Tomazinho  1 , Flares Baratto-Filho  2 , João A Brancher  1 , Luiz F Pereira  3 , Marilisa C L Gabardo  1
Affiliations

Biocompatibility of the 8.25% sodium hypochlorite irrigant solution in endodontics: An in vivo study

Luciana S Demenech et al. Microsc Res Tech. 2021 Jul.

Abstract

The biocompatibility of irrigant solutions, including 8.25% sodium hypochlorite (NaOCl), was analyzed in samples (n = 25) of chorioallantoic membranes (CAM) from embryos of the Gallus gallus, using the hen's egg test on chorioallantoic membrane (HET-CAM). The following irrigants were tested: saline (control), 2% chlorhexidine (2% CHX), and NaOCl (2.5%, 5.25%, and 8.25%), and morphological and microcirculatory changes were investigated. Images were selected and quantified with Image J®. The macroscopic data were submitted to ANOVA, followed by the Tukey's test (p < .05); non-parametric Kruskal-Wallis and Mann-Whitney tests were applied to histological data (p < .05). The frequency of hyperemia differed significantly in the 8.25% NaOCl compared to the other groups, with the exception of 5.25% NaOCl (p = .096). Coagulation was more frequent in the 2% CHX group, differing significantly from the 2.5% NaOCl group (p = .038). In mean values, NaOCl 8.25% caused the decrease (NaOCl = 2.60, and control = 5.00) of vessels in the microcirculation, as well as triggered the increase (NaOCl = 425.50, and control 44.50) of inflammatory cells in the observed areas. There was necrosis in all samples in the 5.25% NaOCl group and in 80% in the 2% CHX, 2.5% NaOCl, and 8.25% NaOCl groups. Fibroblasts were detected only in the control group. It was concluded that the 8.25% NaOCl solution showed significantly high toxicity on CAM. However, these results were comparable to the other concentrations of NaOCl and to 2% CHX. Thus, in this experimental protocol, 8.25% NaOCl proved to be viable as an irrigant solution.

Keywords: chorioallantoic membrane; irrigants; root canal; sodium hypochlorite.

PubMed Disclaimer

References

REFERENCES

    1. Balbino, C. A., Pereira, L. M., & Curi, R. (2005). Mechanisms involved in wound healing: A revision. Revista Brasileira de Ciências Farmacêuticas, 41(1), 27-51.
    1. Castedo, M., Perfettini, J. L., Roumier, T., Valent, A., Raslova, H., Yakushijin, K., … Kroemer, G. (2004). Mitotic catastrophe constitutes a special case of apoptosis whose suppression entails aneuploidy. Oncogene, 23(25), 4362-4370.
    1. Cruvinel, W. M., Mesquita, D., Jr., Araújo, J. A., Catelan, T. T., de Souza, A. W., da Silva, N. P., & Andrade, L. E. C. (2010). Immune system-part I. Fundamentals of innate immunity with emphasis on molecular and cellular mechanisms of inflammatory response. Revista Brasileira de Reumatologia, 50(4), 434-651.
    1. Cullen, J. K., Wealleans, J. A., Kirkpatrick, T. C., & Yaccino, J. M. (2015). The effect of 8.25% sodium hypochlorite on dental pulp dissolution and dentin flexural strength and modulus. Journal of Endodontics, 41(6), 920-924.
    1. Derouiche, M. T. T., & Abdennour, S. (2017). HET-CAM test. Application to shampoos in developing countries. Toxicology in Vitro, 45(3), 393-396.

LinkOut - more resources