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. 2021 Apr;35(4):e14226.
doi: 10.1111/ctr.14226. Epub 2021 Feb 5.

Graft infusion of adipose-derived mesenchymal stromal cells to prevent rejection in experimental intestinal transplantation: A feasibility study

Ane M Andres  1   2   3 , Pablo Stringa  4 , Paloma Talayero  5   6 , Monica Santamaria  7 , Mariano García-Arranz  8 , Soledad García Gómez-Heras  9 , Carlota Largo-Aramburu  10 , Rosa M Aras-Lopez  10 , Maria Teresa Vallejo-Cremades  11 , Laura Guerra Pastrián  12 , Luz Vega  8 , Jose Luis Encinas  1 , Manuel Lopez-Santamaria  1 , Francisco Hernández-Oliveros  3   8   13
Affiliations

Graft infusion of adipose-derived mesenchymal stromal cells to prevent rejection in experimental intestinal transplantation: A feasibility study

Ane M Andres et al. Clin Transplant. 2021 Apr.

Erratum in

  • ERRATUM.
    [No authors listed] [No authors listed] Clin Transplant. 2021 Jun;35(6):e14343. doi: 10.1111/ctr.14343. Epub 2021 May 18. Clin Transplant. 2021. PMID: 34003529 No abstract available.

Abstract

Background: Mesenchymal stromal cells (MSC) have been proposed as a promising complement to standard immunosuppression in solid organ transplantation because of their immunomodulatory properties. The present work addresses the role of adipose-derived MSC (Ad-MSC) in an experimental model of acute rejection in small bowel transplantation (SBT).

Material/methods: Heterotopic allogeneic SBT was performed. A single dose of 1.5x106 Ad-MSC was intra-arterially delivered just before graft reperfusion. Animals were divided into CONTROL (CTRL), CONTROL+Ad-MSC (CTRL_MSC), tacrolimus (TAC), and TAC+Ad-MSC (TAC_MSC) groups. Each Ad-MSC groups was subdivided in autologous and allogeneic third-party groups.

Results: Rejection rate and severity were similar in MSC-treated and untreated animals. CTRL_MSC animals showed a decrease in macrophages, T-cell (CD4, CD8, and Foxp3 subsets) and B-cell counts in the graft compared with CTRL, this decrease was attenuated in TAC_MSC animals. Pro- and anti-inflammatory cytokines and some chemokines and growth factors increased in CTRL_MSC animals, especially in the allogeneic group, whereas milder changes were seen in the TAC groups.

Conclusion: Ad-MSC did not prevent rejection when administered just before reperfusion. However, they showed immunomodulatory effects that could be relevant for a longer-term outcome. Interference between tacrolimus and the MSC effects should be addressed in further studies.

Keywords: adipose-derived mesenchymal stromal cells; rejection; small bowel transplantation.

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References

REFERENCES

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