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Comment
. 2021 Apr;77(4):e42-e43.
doi: 10.1161/HYPERTENSIONAHA.120.16795. Epub 2021 Jan 20.

Angiotensin Type 1 Receptor-Dependent Internalization of SARS-CoV-2 by Angiotensin-Converting Enzyme 2

Blessing O Ogunlade  1 , Eric Lazartigues  2   3   4 , Catalin M Filipeanu  1
Affiliations
Comment

Angiotensin Type 1 Receptor-Dependent Internalization of SARS-CoV-2 by Angiotensin-Converting Enzyme 2

Blessing O Ogunlade et al. Hypertension. 2021 Apr.
No abstract available

Keywords: angiotensin II; angiotensin-converting enzyme 2; cardiovascular diseases; coronavirus; receptor, angiotensin, type 1.

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Figures

Figure.
Figure.
The effects of Ang II (angiotensin II) and S1 spike protein (S1) on ACE2 (angiotensin-converting enzyme 2) in HEK293T cells. A, Subcellular localization of ACE2 (green) in HEK293T cells cotransfected with 0.5 μg GFP (green fluorescent protein)-tagged ACE2 and 1 μg pcDNA3.1 (control vector, top) or HA-tagged AT1R (angiotensin type 1 receptor; bottom) after vehicle (left), Ang II (1 μmol/L, middle) or S1 (30 ng/mL, right) treatment for 4 h. B, ACE2 activity in HEK293T cells cotransfected with GFP-tagged ACE2 (0.5 μg) and HA-tagged AT1R (0, 0.5, and 1 μg). The total transfected cDNA was kept at 1.5 μg by addition of necessary amounts of pcDNA3.1. ACE2 activity was determined in vehicle-treated cells or after 4 h (corresponding to maximal effect on enzyme activity) treatment with Ang II (1 μmol/L) or S1 (30 ng/mL). C, Pretreatment (30 min) with the AT1R antagonist losartan (1 μmol/L,) or β-arrestin biased agonist TRV027 (100 nmol/L) blocked Ang II– or S1-mediated reduction of ACE2 activity in HEK293T cells transfected with 0.5 μg GFP-ACE2 and 1 μg AT1R. D, Effects of treatment for 18 h (corresponding to maximal effect on protein levels) with Ang II (1 μmol/L) or S1 (30 ng/mL) on total ACE2 cellular levels determined by Western blotting. E, Pretreatment (30 min each) with losartan (1 μmol/L), HCQ (hydroxychloroquine; 100 μmol/L) or sucrose (100 mmol/L) blocks the decrease of ACE2 activity induced by Ang II (1 μmol/L) or S1 (30 ng/mL). The data are presented as mean ±SEM with n=3–4 from at least 3 independent transfections. The experiments were performed as described previously5 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456754/).
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References

    1. Lan J, Ge J, Yu J, Shan S, Zhou H, Fan S, Zhang Q, Shi X, Wang Q, Zhang L, et al. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature. 2020; 581:215–220. doi: 10.1038/s41586-020-2180-5 - PubMed
    1. Danser AHJ, Epstein M, Batlle D. Renin-angiotensin system blockers and the COVID-19 pandemic: at present there is no evidence to abandon renin-angiotensin system blockers. Hypertension. 2020; 75:1382–1385. doi: 10.1161/HYPERTENSIONAHA.120.15082 - PMC - PubMed
    1. Deshotels MR, Xia H, Sriramula S, Lazartigues E, Filipeanu CM. Angiotensin II mediates angiotensin converting enzyme type 2 internalization and degradation through an angiotensin II type I receptor-dependent mechanism. Hypertension. 2014; 64:1368–1375. doi: 10.1161/HYPERTENSIONAHA.114.03743 - PMC - PubMed
    1. Carvalho-Galvão A, Ogunlade B, Xu J, Silva-Alves CRA, Mendes-Júnior LG, Guimarães DD, Cruz JC, Queiroz TM, Balarini CM, Braga VA, et al. Central administration of TRV027 improves baroreflex sensitivity and vascular reactivity in spontaneously hypertensive rats. Clin Sci (Lond). 2018; 132:1513–1527. doi: 10.1042/CS20180222 - PMC - PubMed
    1. Ogunlade B, Guidry JJ, Mukerjee S, Sriramula S, Lazartigues E, Filipeanu CM. The actin bundling protein fascin-1 as an ACE2-accessory protein [published online August 31, 2020]. Cell Mol Neurobiol. doi: 10.1007/s10571-020-00951-x. https://link.springer.com/article/10.1007/s10571-020-00951-x - DOI - PMC - PubMed

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