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. 2021 May;19(5):863-865.
doi: 10.1111/pbi.13552. Epub 2021 Feb 5.

Mutagenesis of UDP-xylose epimerase and xylan arabinosyl-transferase decreases arabinose content and improves saccharification of rice straw

Chen Chen  1   2 , Xianhai Zhao  1   2 , Xuchuan Wang  1   2 , Bo Wang  3 , Huiling Li  1   2 , Jiaxun Feng  4 , Aimin Wu  1   2   5
Affiliations

Mutagenesis of UDP-xylose epimerase and xylan arabinosyl-transferase decreases arabinose content and improves saccharification of rice straw

Chen Chen et al. Plant Biotechnol J. 2021 May.
No abstract available

Keywords: OsUXE; OsXAT; CRISPR; Cas9; biomass feedstock; cell wall; rice.

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Conflict of interest statement

No conflict of interests to declare.

Figures

Figure 1
Figure 1
The cell wall changes of uxe and xat mutants improve the ability of straw to be hydrolysed. (a) The mutation site of the mutant (by CRISPR/Cas 9). Red markers represent mutations and dashed lines represent missing bases. (b) Schematic representation of targets in genes. Block shows exons and coloured section indicates target position. Line represents introns. (c) Photographs of plant growth. (d) Cross sectioning of rice stems. Toluidine blue dye was used. (e) Determination of amounts of arabinose, xylose and glucose in plant stem cell wall. Results are shown as ratio of monosaccharides to cell wall weight. (f) Enzyme activity of UXEs among uxes mutants and WT by extracted microsome with method referenced by Gu et al. (https://doi.org/10.1099/mic.0.040758‐0). (g) Growth condition statistics of mutants in the field. (h) 2D‐HSQC comparative of hemicellulose. Abbreviations: A, Araf; X, Xylp; (i) determination of amount of lignin of plant stem cell wall by pyGC‐MS. S (%) and G (%) data indicate proportion of syringyl units and guaiacyl units in total composition, respectively. (j) Enzymatic hydrolysis efficiency of cellulose in rice mutants. (k) XRD of uxes and xats. Scanning speed: 5°/min. Three biological replicates were used as test samples, and error bars are based on three data results. Asterisk indicates that sample is significant compared to WT (‘*’, P < 0.05; ‘**’, P < 0.01; Student's t‐test).
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References

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