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. 2021 Mar 19;59(4):e02252-20.
doi: 10.1128/JCM.02252-20. Print 2021 Mar 19.

Genetic Evaluation of Nosocomial Candida auris Transmission

Scott C Roberts  1 , Teresa R Zembower  2   3 , Egon A Ozer #  2 , Chao Qi #  4
Affiliations

Genetic Evaluation of Nosocomial Candida auris Transmission

Scott C Roberts et al. J Clin Microbiol. .

Abstract

Whole-genome sequences of Candida auris isolates from nosocomial and nonnosocomial infections were compared. The average numbers of single nucleotide variations were different between the two groups. The small amount of genetic variability between intra- or interhost isolates suggests recovery of all colonizing or infecting genomes for comparison is required for outbreaks.

Keywords: Candida auris; nosocomial transmission; whole-genome sequencing.

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Figures

FIG 1
FIG 1
(A) Phylogenetic analysis of NMH and ACL isolates. Midpoint-rooted maximum likelihood phylogenetic analysis of all NMH and ACL isolates with corresponding fluconazole sensitivity profiles, when known. The first highlighted nodes represent four isolates from a single patient. The second highlighted nodes represent environmental strains from the room of patient CA11. Nosocomial strains are CA01, CA04, CA06, CA07, and CA12. (B) Pairwise SNV differences between nosocomial and POA isolates. N, nosocomial isolates; P, isolates from POA patients, ns, no significant difference. Statistical analysis was performed with unpaired Wilcoxon tests. **, P < 0.01; ***, P < 0.001. (C) Pairwise SNP differences between NMH and ACL strains. ACL, isolates from ACL Laboratories; NMH, isolates from NMH nosocomial and POA patients; ns, no significant difference. Statistical analysis was performed with unpaired Wilcoxon tests.
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