doi: 10.1128/jb.132.1.349-351.1977.
Transformation in Escherichia coli: cryogenic preservation of competent cells
- PMID: 334731
- PMCID: PMC221863
- DOI: 10.1128/jb.132.1.349-351.1977
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Transformation in Escherichia coli: cryogenic preservation of competent cells
J Bacteriol.
1977 Oct.
Abstract
Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.
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