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. 1977 Oct;132(1):349-51.
doi: 10.1128/jb.132.1.349-351.1977.

Transformation in Escherichia coli: cryogenic preservation of competent cells

D A Morrison

Transformation in Escherichia coli: cryogenic preservation of competent cells

D A Morrison. J Bacteriol. 1977 Oct.

Abstract

Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.

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References

    1. Virology. 1955 Jul;1(2):190-206 - PubMed
    1. J Bacteriol. 1964 Dec;88:1538-44 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Aug;52:412-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3838-42 - PubMed
    1. J Bacteriol. 1969 Mar;97(3):1033-5 - PubMed

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