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. 1977 Oct;132(1):359-61.
doi: 10.1128/jb.132.1.359-361.1977.

Localized mutagenesis with bacteriophage Mu: method for increasing the frequency of specific bacterial mutants

H Tabor, E W Hafner, C W Tabor

Localized mutagenesis with bacteriophage Mu: method for increasing the frequency of specific bacterial mutants

H Tabor et al. J Bacteriol. 1977 Oct.

Abstract

A method is described for markedly enriching a bacterial population for cells containing any given Mu insertion mutation. The method involves the transfer of a small piece of deoxyribonucleic acid from a Mu-infected Hfr donor donor strain to a suitable F- strain and a subsequent selection of those recombinant organisms that have received a Mu prophage from the donor. The method is particularly usefule for isolating mutants whose selection requires "brute-force" assay, since only a few hundred colonies have to be screened.

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