The two complete plastomes from Scrophularia (Scrophulariaceae): Scrophularia buergeriana and S. takesimensis

Abstract

The plastome sequences of Scrophularia buergeriana and S. takesimensis are completed in family Scrophulariaceae. The structure of two Scrophularia plastomes shows similar characteristic with the typical plastome of angiosperm. The lengths of two plastomes are 153,631bp and 152,436bp, respectively. They are divided into LSC region (84,454bp and 83,542bp) and SSC region (17,929bp and 17,938bp) by two IR regions (25,624bp and 25,478bp). Both plastomes contain 113 genes including 79 protein coding genes, 30 tRNA genes and 4 rRNA genes. Eight protein-coding, seven tRNA and four rRNA genes are duplicated in the IR regions. Eighteen genes have one or two intron(s). The overall A-T contents of two genomes are 62.0% and 61.9%, respectively. The A-T content in the non-coding (both 64.5%) is higher than in the coding (60.2% and 60.1%) region. Forty-four and forty-one simple sequence repeat (SSR) loci are identified in the S. buergeriana and S. takesimensis plastomes, respectively. In phylogenetic analysis, the genus Scrophularia shows closed relationship with Plantaginaceae.

Keywords: Scrophularia buergeriana; Scrophularia takesimensis; Scrophulariaceae; plastome.

Figure 1.

Chloroplast phylogenetic tree of Lamiales. A maximum-likelihood tree (−lnL= 459,584.0962) inferred from analysis of alignment data containing 79 coding genes in 56 chloroplast genome sequences by use of the GTR + Γ+I model. The numbers above and below each node indicate the Bayesian support percentages and bootstrap value, respectively. Genbank accession numbers for each taxa are Ajuga reptans (NC_023102), Andrographis paniculata (NC_022451), Boulardia latisquama (NC_025641), Cistanche deserticola (NC_021111), C. phelypaea (NC_025642), Coffea arabica (NC_008535), C. canephora (NC_030053), Conopholis americana (NC_023131), Dorcoceras hygrometricum (NC_016468), Epifagus virginiana (NC_001568), Genlisea margaretae (NC_025652), Haplostachys haplostachya (NC_029819), Hesperelaea palmeri (NC_025787), Jasminum nudiflorum (NC_008407), Lathraea squamaria (NC_027838).