The complete mitochondrial genome of the Basidiomycete edible fungus Pleurotus eryngii

Abstract

The complete mitochondrial genome of the edible fungus Pleurotus eryngii (oyster mushroom) was determined using Illumina sequencing. This mitogenome is a circular molecule of 72,650 bp in length with a GC content of 26.28%. Gene prediction showed that 40 putative protein-coding genes, the small ribosomal RNA subunits (rns), and 23 tRNAs were located on the same strand. The mitogenome of P. eryngii has a similar structure to that of P. ostreatus in both gene content and gene order. The mitogenome information of P. eryngii should contribute to our understanding of the diversity and evolution of Pleurotaceae and Agaricales.

Keywords: Pleurotus eryngii; agaricales; mitochondrial genome; pleurotaceae.

Figure 1.

Phylogenetic analysis of 19 species of Agaricomycotina conducted by Neighbour-joining method as implemented in MEGA 7.0 (Tokyo, Japan, Kumar et al. 2016) based on concatenated amino acid sequences of 12 mitochondrial protein-coding genes, including atp8, atp9, cob, cox1, cox2, cox3, nad1, nad2, nad3, nad4L, nad5 and nad6. The concatenated sequences were aligned using Clustal X (Thompson et al. 2010). The 19 species belonged to Agaricomycotina used for phylogenetics were listed below: Cantharellus cibarius (NC_020368), Flammulina velutipes (NC_021373), Ganoderma applanatum (NC_027188), Ganoderma lucidum (NC_021750), Ganoderma meredithae (NC_026782), Ganoderma sinense (NC_022933), Heterobasidion irregulare (NC_024555), Lentinula edodes (NC_018365), Moniliophthora perniciosa (NC_005927), Moniliophthora roreri (NC_015400), Phlebia radiata (NC_020148), Pleurotus ostreatus (NC_009905), Rhizoctonia solani (HF546977), Schizophyllum commune (NC_003049), Serendipita indica (FQ859090), Trametes cingulata (NC_013933) and Tricholoma matsutake (NC_028135). Neurospora crassa (NC_026614) was served as outgroup. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) were shown next to the branches.