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. 2018 Sep 28;3(2):1000-1001.
doi: 10.1080/23802359.2018.1507643.

Characterization of the complete chloroplast genome of Lonicera macranthoides

Huan Hu  1 , Jianguo Liu  1 , Jiaxing An  2 , Miao Wang  1 , Qian Wang  1
Affiliations

Characterization of the complete chloroplast genome of Lonicera macranthoides

Huan Hu et al. Mitochondrial DNA B Resour. .

Abstract

Lonicera macranthoides is an important Chinese traditional medicine plant which is endemic in southwest China. The complete chloroplast genome from L. macranthoides is determined in this study. The genome size was 154,897 bp, containing a large single copy (LSC) region of 88,692 bp and a small single copy (SSC) region of 18,623 bp, which were separated by a pair of 23,786 bp inverted repeat (IR) regions. The plastome contained 130 genes, including 81 protein-coding genes (77 PCG species), eight ribosomal RNA genes (4 rRNA species), 39 tRNA genes (29 tRNA species) and two pseudognes. The most of gene species occurred as a single copy, while 13 gene species occurred in double copies, including all rRNA species, six tRNA species and three PCG species, and two tRNA genes occur in treble copies. Finally, phylogenetic analysis demonstrated that L. macranthoides is closely related to L. japonica, and genus Lonicera shows a closer relationship with Triostrum for the current data.

Keywords: Caprifoliaceae; Chloroplast genome; Lonicera macranthoides; Phylogenetic analysis.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Neighbor-joining (NJ) tree based on the whole chloroplast genome sequences of 12 taxa including Lonicera macranthoides and one outgroup taxon. The whole chloroplast genome sequences were aligned using MAFFT online version (https://mafft.cbrc.jp/alignment/server/) and subjected to generating NJ phylogenetic tree by MEGA v7.0 (Kumar et al. 2016). The bootstrap support values (>50%) from 1,000 replicates are indicated in the nodes. Chloroplast genome sequences used for this tree are: Lonicera macranthoides, MH579750; Lonicera japonica, KJ170923; Lonicera stephanocarpa, MG738668; Lonicera frangrantissima var. lancifolia, MG738669; Lonicera tragophylla, MG738667; Triosteum pinatifidum, MG738666; Patrinia saniculifolia, MG517444; Dipelta floribunda, MG738670; Kolkwitzia amabilis, KT966716; Weigela florida, MG738664; Sinadoxa corydalifolia, KX258651 (outgroup).
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