Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May;230(3):972-987.
doi: 10.1111/nph.17221. Epub 2021 Feb 25.

The Arabidopsis condensin CAP-D subunits arrange interphase chromatin

Celia Municio  1 , Wojciech Antosz  2 , Klaus D Grasser  2 , Etienne Kornobis  3   4 , Michiel Van Bel  5 , Ignacio Eguinoa  5 , Frederik Coppens  5 , Andrea Bräutigam  1 , Inna Lermontova  1   6 , Astrid Bruckmann  7 , Katarzyna Zelkowska  1 , Andreas Houben  1 , Veit Schubert  1
Affiliations
Free article

The Arabidopsis condensin CAP-D subunits arrange interphase chromatin

Celia Municio et al. New Phytol. 2021 May.
Free article

Abstract

Condensins are best known for their role in shaping chromosomes. Other functions such as organizing interphase chromatin and transcriptional control have been reported in yeasts and animals, but little is known about their function in plants. To elucidate the specific composition of condensin complexes and the expression of CAP-D2 (condensin I) and CAP-D3 (condensin II), we performed biochemical analyses in Arabidopsis. The role of CAP-D3 in interphase chromatin organization and function was evaluated using cytogenetic and transcriptome analysis in cap-d3 T-DNA insertion mutants. CAP-D2 and CAP-D3 are highly expressed in mitotically active tissues. In silico and pull-down experiments indicate that both CAP-D proteins interact with the other condensin I and II subunits. In cap-d3 mutants, an association of heterochromatic sequences occurs, but the nuclear size and the general histone and DNA methylation patterns remain unchanged. Also, CAP-D3 influences the expression of genes affecting the response to water, chemicals, and stress. The expression and composition of the condensin complexes in Arabidopsis are similar to those in other higher eukaryotes. We propose a model for the CAP-D3 function during interphase in which CAP-D3 localizes in euchromatin loops to stiffen them and consequently separates centromeric regions and 45S rDNA repeats.

Keywords: Arabidopsis thaliana; SMC proteins; chromatin organization; chromosomes; condensin; interphase nuclei.

PubMed Disclaimer

References

    1. Afgan E, Baker D, Batut B, Van Den Beek M, Bouvier D, Čech M, Chilton J, Clements D, Coraor N, Grüning BA et al. 2018. The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Research 46: W537-W544.
    1. Anders S, Pyl PT, Huber W. 2015. HTSeq - a Python frame work to work with high-throughtput sequencing data. Bioinformatics 31: 166-169.
    1. Andrey P, Kieu K, Kress C, Lehmann G, Tirichine L, Liu ZC, Biot E, Adenot PG, Hue-Beauvais C, Houba-Herin N et al. 2010. Statistical analysis of 3D images detects regular spatial distributions of centromeres and chromocenters in animal and plant nuclei. PLoS Computational Biology 6: e1000853.
    1. Antosz W, Pfab A, Ehrnsberger HF, Holzinger P, Kollen K, Mortensen SA, Bruckmann A, Schubert T, Langst G, Griesenbeck J et al. 2017. The composition of the Arabidopsis RNA polymerase II transcript elongation complex reveals the interplay between elongation and mRNA processing factors. The Plant Cell 29: 854-870.
    1. Bauer CR, Hartl TA, Bosco G. 2012. Condensin II promotes the formation of chromosome territories by inducing axial compaction of polyploid interphase chromosomes. PLoS Genetics 8: e1002873.

Publication types

LinkOut - more resources