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. 2021 Feb;48(2):1233-1241.
doi: 10.1007/s11033-021-06135-0. Epub 2021 Jan 21.

Influence of microcurrent on the modulation of remodelling genes in a wound healing assay

Daniela Fernanda Dezotti Silva  1 , Lucas de Oliveira Fujii  1 , Gabriela Bortolança Chiarotto  1 , Camila Andrea de Oliveira  1 , Thiago Antônio Moretti de Andrade  1 , Alexandre Leite Rodrigues de Oliveira  2 , Marcelo Augusto Marretto Esquisatto  1 , Fernanda Aparecida Sampaio Mendonça  1 , Gláucia Maria Tech Dos Santos  1 , Andrea Aparecida de Aro  3   4
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Influence of microcurrent on the modulation of remodelling genes in a wound healing assay

Daniela Fernanda Dezotti Silva et al. Mol Biol Rep. 2021 Feb.

Abstract

The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb), connective tissue growth factor (Ctgf), insulin-like growth factor 1 (Igf1), tenascin C (Tnc), Fibronectin (Fn1), Scleraxis (Scx), Fibromodulin (Fmod) and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell migration was analyzed between days 0 and 4 in both fibroblasts (F) and fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene expression were also analyzed after daily MC application. Higher expression of Ctgf and lower expression of Tnc and Fmod, respectively, were observed in the F+MC group in relation to F group (p < 0.05), and no difference was observed between the groups for the genes Tgfb, Fn1 and Scx. In cell migration, a higher number of cells in the scratch region was observed in group F+MC (p < 0.05) compared to group F on the 4th day, and the cell viability assay showed no difference between the groups. In conclusion, MC therapy at an intensity/time of 10 μA/90 s with 4 daily applications did not affect cell viability, stimulated fibroblasts migration with the involvement of Ctgf, and reduced the Tnc and Fmod expression.

Keywords: Cell migration; Fibroblasts; Genes; Microcurrent; Therapy.

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