Loop-mediated isothermal amplification (LAMP): An effective molecular point-of-care technique for the rapid diagnosis of coronavirus SARS-CoV-2

Abstract

The novel coronavirus disease-2019 (Covid-19) public health emergency has caused enormous loss around the world. This pandemic is a concrete example of the existing gap between availability of advanced diagnostics and current need for cost-effective methodology. The advent of the loop-mediated isothermal amplification (LAMP) assay provided an innovative tool for establishing a rapid diagnostic technique based on the molecular amplification of pathogen RNA or DNA. In this review, we explore the applications, diagnostic effectiveness of LAMP test for molecular diagnosis and surveillance of severe acute respiratory syndrome coronavirus 2. Our results show that LAMP can be considered as an effective point-of-care test for the diagnosis of Covid-19 in endemic areas, especially for low- and middle-income countries.

Keywords: Covid-19; POC; SARS-CoV-2; coronavirus; diagnostic; loop-mediated isothermal amplification (LAMP).

FIGURE 1

Schematic principle of loop‐mediated isothermal amplification

FIGURE 2

Evolution of the number of Covid‐19 loop‐mediated isothermal amplification studies

FIGURE 3

Country origin of loop‐mediated isothermal amplification assays studies

FIGURE 4

Proportion of targeted genes used for loop‐mediated isothermal amplification development studies