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Review
. 2021 Nov;31(6):e2215.
doi: 10.1002/rmv.2215. Epub 2021 Jan 21.

Loop-mediated isothermal amplification (LAMP): An effective molecular point-of-care technique for the rapid diagnosis of coronavirus SARS-CoV-2

Melek Chaouch  1   2
Affiliations
Review

Loop-mediated isothermal amplification (LAMP): An effective molecular point-of-care technique for the rapid diagnosis of coronavirus SARS-CoV-2

Melek Chaouch. Rev Med Virol. 2021 Nov.

Abstract

The novel coronavirus disease-2019 (Covid-19) public health emergency has caused enormous loss around the world. This pandemic is a concrete example of the existing gap between availability of advanced diagnostics and current need for cost-effective methodology. The advent of the loop-mediated isothermal amplification (LAMP) assay provided an innovative tool for establishing a rapid diagnostic technique based on the molecular amplification of pathogen RNA or DNA. In this review, we explore the applications, diagnostic effectiveness of LAMP test for molecular diagnosis and surveillance of severe acute respiratory syndrome coronavirus 2. Our results show that LAMP can be considered as an effective point-of-care test for the diagnosis of Covid-19 in endemic areas, especially for low- and middle-income countries.

Keywords: Covid-19; POC; SARS-CoV-2; coronavirus; diagnostic; loop-mediated isothermal amplification (LAMP).

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Conflict of interest statement

The author declares that there is no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Schematic principle of loop‐mediated isothermal amplification
FIGURE 2
FIGURE 2
Evolution of the number of Covid‐19 loop‐mediated isothermal amplification studies
FIGURE 3
FIGURE 3
Country origin of loop‐mediated isothermal amplification assays studies
FIGURE 4
FIGURE 4
Proportion of targeted genes used for loop‐mediated isothermal amplification development studies
See this image and copyright information in PMC

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Supplementary concepts