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Review
. 2021 Jan 18;10(1):128.
doi: 10.3390/antiox10010128.

Comprehensive Review of Methodology to Detect Reactive Oxygen Species (ROS) in Mammalian Species and Establish Its Relationship with Antioxidants and Cancer

Shivkanya Fuloria  1 , Vetriselvan Subramaniyan  2 , Sundram Karupiah  1 , Usha Kumari  3 , Kathiresan Sathasivam  4 , Dhanalekshmi Unnikrishnan Meenakshi  5 , Yuan Seng Wu  2 , Mahendran Sekar  6 , Nitin Chitranshi  7 , Rishabha Malviya  8 , Kalvatala Sudhakar  9 , Sakshi Bajaj  10 , Neeraj Kumar Fuloria  1
Affiliations
Review

Comprehensive Review of Methodology to Detect Reactive Oxygen Species (ROS) in Mammalian Species and Establish Its Relationship with Antioxidants and Cancer

Shivkanya Fuloria et al. Antioxidants (Basel). .

Abstract

Evidence suggests that reactive oxygen species (ROS) mediate tissue homeostasis, cellular signaling, differentiation, and survival. ROS and antioxidants exert both beneficial and harmful effects on cancer. ROS at different concentrations exhibit different functions. This creates necessity to understand the relation between ROS, antioxidants, and cancer, and methods for detection of ROS. This review highlights various sources and types of ROS, their tumorigenic and tumor prevention effects; types of antioxidants, their tumorigenic and tumor prevention effects; and abnormal ROS detoxification in cancer; and methods to measure ROS. We conclude that improving genetic screening methods and bringing higher clarity in determination of enzymatic pathways and scale-up in cancer models profiling, using omics technology, would support in-depth understanding of antioxidant pathways and ROS complexities. Although numerous methods for ROS detection are developing very rapidly, yet further modifications are required to minimize the limitations associated with currently available methods.

Keywords: antioxidants; detection methods; harmful and beneficial effects; reactive oxygen species.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Various types of reactive oxygen species (ROS) and their sources.
Figure 2
Figure 2
Various types of endogenous (EnAO) and exogenous (ExAO) antioxidants.
Figure 3
Figure 3
Role of NRF2 at various of cancer stages.
See this image and copyright information in PMC

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