Microbacterium karelineae sp. nov. isolated from a halophyte plant in the Taklamakan desert

Abstract

A bacterial strain, designated TRM 80801^T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain TRM 80801^T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801^T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306^T (98.97 %) and Microbacterium indicum BBH6^T (98.17 %), respectively. The DNA G+C content of TRM 80801^T is 69.38 mol%. The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine. The acyl type of the peptidoglycan was glycolyl. Whole-cell sugars were ribose, mannose, glucose, rhamnose and galactose. The major cellular fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and iso-C_{16 : 0}. The predominant menaquinones were MK-10, MK-11 and MK-12. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol. The whole-genome average nucleotide identity (ANI) value between strain TRM 80801^T and Microbacterium suaedae YZYP 306^T is 70.2 %. On the basis of the evidence presented in this study, strain TRM 80801^T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp. nov. is proposed. The type strain is TRM 80801^T (=CCTCC AB 2019248^T=KCTC 49357^T).

Keywords: Actinomycetes; Microbacterium karelineae; Taklamakan desert.